PRMT6 Antibody

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N-methyltransferase (PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42 kDa consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG-monomethylarginine and asymmetric >NG,NG-dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activit. GFP-fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NB100-56642	Applications	Species
Khalil M, Ismail H, Panasyuk G et al. Asymmetric Dimethylation of Ribosomal S6 Kinase 2 Regulates Its Cellular Localisation and Pro-Survival Function International journal of molecular sciences 2023-05-15 [PMID: 37240151]
Wang Z, Pan Z, Adhikari S, et al. m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region The EMBO journal 2021-01-18 [PMID: 33459381]
Zhang H, Han C, Li T et al. The methyltransferase PRMT6 attenuates antiviral innate immunity by blocking TBK1-IRF3 signaling. Cell. Mol. Immunol. 2018-07-04 [PMID: 29973649]
Di Lorenzo A. Gain-of-function Mouse Models to Investigate Biological Roles of PRMT6. Thesis 2014-01-01 (WB, Human)	WB	Human
Kim JK, Lim Y, Lee JO et al. PRMT4 is involved in insulin secretion via methylation of histone H3 in pancreatic beta-cells J. Mol. Endocrinol. 2015-04-27 [PMID: 25917831] (WB, Human) Details: PRMT6 antibody was used for WB analysis of lysates from pancreatic beta-cell line/HIT-T15 b cells which was pre-incubated in KRBH buffer without glucose for 1 h and then stimulated for different time intervals with 16.7 mM glucose in KRBH buffer (Figure 1B).	WB	Human
Lorenzo AD, Yang Y, Macaluso M, Bedford MT. Gain-of-function mouse models to investigated biological roles of PRMT6. Nucleic Acids Res. 2014-06-17 [PMID: 24939901] (WB, Mouse, Human) Details: Fig 16B, human-PRMT6 transgenic &WT mouse embryo fibroblasts. The specificity of the PRMT6 was validated in PRMT6 transgenic cells by WB (Fig 16B).	WB	Mouse, Human
Obianyo O, Causey CP, Jones JE, Thompson PR. Activity-based protein profiling of protein arginine methyltransferase 1. ACS Chem Biol. 2011-10-21 [PMID: 21838253] (WB) Details: WB (MCF-7), Fig 4B.	WB
Lim Y, Hong E, Kwon D et al. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis. 2010-12-01 [PMID: 21080485] (WB) Details: WB: (immortalized WI-38 VA13 fibroblasts, normal young WI-38 fibroblasts, fully senescent WI-38 fibroblasts), Fig 1A, B. Note: Fully senescent cells were obtained by routinely subculturing young fibroblasts and then maintaining the replicatively growth-ar	WB
Kim C, Lim Y, Yoo BC et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta. 2010-09-01 [PMID: 20541591] (WB) Details: WB (HeLa), Fig 2A	WB
Yu Z, Chen T, Hebert J et al. A mouse PRMT1 null allele defines an essential role for arginine methylation in genome maintenance and cell proliferation. Mol Cell Biol. 2009-06-01 [PMID: 19289494] (WB, Mouse) Details: WB (mouse embryonic fibroblasts), Fig. 2E.	WB	Mouse
Herrmann F, Pably P, Eckerich C et al. Human protein arginine methyltransferases in vivo--distinct properties of eight canonical members of the PRMT family. J Cell Sci. 2009-03-01 [PMID: 19208762] (WB) Details: 1. IMG-496 (PRMT4): WB (HEk 293 cells), Fig. 1C,B. 2. IMG-506 (PRMT6): WB (HEk 293 cells), Fig. 1C,B.	WB
Lim Y, Lee E, Lee J et al. Down-regulation of asymmetric arginine methylation during replicative and H2O2-induced premature senescence in WI-38 human diploid fibroblasts. J Biochem. 2008-10-01 [PMID: 18676353] (WB, Human) Details: WB (WI-38 normal human diploid fibroblasts), Fig. 1D, 2D.	WB	Human
Fronz K, Otto S, Kolbel K et al. Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior. J Biol Chem. 2008-07-18 [PMID: 18495660] (WB) Details: WB(HeLa cells), Fig. 5.	WB
Kitowska K, Zakrzewicz D, K0nigshoff M et al. Functional role and species-specific contribution of arginases in pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2008-01-01 [PMID: 17934065]
Guccione E, Bassi C, Casadio F et al. Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive. Nature. 2007-10-18 [PMID: 17898714] (WB) Details: WB [Fig. 2a (PRMT6 SiRNA transfected HeLa cells), 2b (PRMT6 transfected HeLa cells), Sup Fig. 6 (PRMT6 SiRNA transfected HeLa cells)]. The antibody was SiRNA validated in Fig. 2a and PRMT6-transfected validated by western blot in Fig. 2b. Endogenous level	WB
Xie B, Invernizzi CF, Richard S, Wainberg MA. Arginine methylation of the human immunodeficiency virus type 1 Tat protein by PRMT6 negatively affects Tat Interactions with both cyclin T1 and the Tat transactivation region. J Virol. 2007-04-01 [PMID: 17267505] (WB) Details: WB (Fig 5), HeLa, Jurkat, and 293T cells), SiRNA validated (Fig 5, in HeLa, Jurkat, and 293T cells).	WB
Bulau P, Zakrzewicz D, Kitowska K et al. Analysis of methylarginine metabolism in the cardiovascular system identifies the lung as a major source of ADMA. Am J Physiol Lung Cell Mol Physiol. 2007-01-01 [PMID: 16891395]
Herrmann F, Lee J, Bedford MT et al. Dynamics of human protein arginine methyltransferase 1(PRMT1) in vivo. J Biol Chem. 2005-11-11 [PMID: 16159886]
Lim Y, Kwon YH, Won NH et al. Multimerization of expressed protein-arginine methyltransferases during the growth and differentiation of rat liver. Biochim Biophys Acta. 2005-05-25 [PMID: 15837430]
Boulanger MC, Liang C, Russell RS et al. Methylation of Tat by PRMT6 regulates human immunodeficiency virus type 1 gene expression. J Virol. 2005-01-01 [PMID: 15596808]
Herrmann F, Bossert M, Schwander A et al. Arginine methylation of scaffold attachment factor A by heterogeneous nuclear ribonucleoprotein particle-associated PRMT1. J Biol Chem. 2004-11-19 [PMID: 15364944]
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	04/04/2020	View Summary Application Western Blot Sample Tested BT-20 human breast cancer cell line,SUM-149 Species Human Lot 111302B-01
Enlarge	5 reviewed by: Verified Customer	WB	Human	12/27/2019	View Summary Application Western Blot Sample Tested bt549 Species Human Lot 111302B-01
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	10/17/2018	View Summary Application Western Blot Sample Tested 4T1 cells whole cell lysate,EMT6 cells Species Mouse Lot 111302B-01

Array NB100-56642

• PRMT6 Antibodies
• PRMT6 Lysates
• PRMT6 Proteins