![Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Western-Blot-NBP2-19939-img0001.jpg "Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary. |
| Localization | Cytoplasm; cytosol; Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRMT7 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ChIP- Assay dependent |
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| Theoretical MW | 78 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19939-img0005.jpg "Immunocytochemistry/Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Immunohistochemistry-Paraffin-NBP2-19939-img0004.jpg "Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.")
![Chromatin Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Chromatin-Immunoprecipitation-NBP2-19939-img0012.jpg "Chromatin Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.")
![Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Immunohistochemistry-Paraffin-NBP2-19939-img0002.jpg "Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Immunohistochemistry-Paraffin-NBP2-19939-img0003.jpg "Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.")
![Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 ug of PRMT7 antibody 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PRMT7-Antibody-Immunoprecipitation-NBP2-19939-img0008.jpg "Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 ug of PRMT7 antibody 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")