MBP Antibody (12)

Images

 
Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 ...read more
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] - Microglia processes appear to preferentially contact TBI-induced proximal axonal swellings. Representative 3D reconstructions of MBP+ myelinated ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC
Clone
12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

MBP Antibody (12) Summary

Immunogen
Bovine MBP
Epitope
Amino acids 82-87.
Localization
Cytoplasmic side of myelin
Marker
Oligodendrocyte Marker, Myelin Marker
Specificity
NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MBP
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunoassay
  • Radioimmunodiffusion
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-717 in the following applications:

Publications
Read Publications using
NB600-717 in the following applications:

Reactivity Notes

Predicted cross-reactivities: Mouse, Rabbit, Porcine, Mammals, Human, Guinea Pig, Chicken, Rat, Sheep

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 M Tris
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MBP Antibody (12)

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBP Antibody (NB600-717)(46)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Canine, Feline, Guinea Pig, Primate.

We have publications tested in 8 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(7)
IHC
(2)
IHC-Fr
(3)
IHC-P
(2)
Immunocytochemistry/ Immunofluorescence
(2)
Immunohistochemistry
(2)
WB
(9)
All Applications
Filter By Species
Human
(2)
Mouse
(14)
Rat
(5)
Bovine
(1)
Canine
(1)
Feline
(1)
Guinea Pig
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NB600-717 Applications Species
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP et Al. Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing Cell 2024-12-07 [PMID: 39644898]
Schubert MC, Soyka SJ, Tamimi A et Al. Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis Nat Commun 2024-09-10 [PMID: 39256378]
Gu X, Rahman FS, Bendale G et al. Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries Research square 2023-11-06 [PMID: 37986821]
Bradbury AM, Bagel J, Swain G et al. Combination HSCT and intravenous AAV-mediated gene therapy in a canine model proves pivotal for translation of Krabbe disease therapy Molecular therapy : the journal of the American Society of Gene Therapy 2023-11-11 [PMID: 37952085]
Kameyama T, Miyata M, Shiotani H et Al. Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain iScience 2023-09-21 [PMID: 37829206]
Lin Q, Hu DW, Hao XH et al. Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat Brains Neural plasticity 2023-04-20 [PMID: 37124874] (WB, Rat) WB Rat
Macintosh J, Michell-Robinson M, Chen X et al. An optimized and validated protocol for the purification of PDGFR?+ oligodendrocyte precursor cells from mouse brain tissue via immunopanning MethodsX 2023-02-01 [PMID: 36814689]
Sohel MSH, Homma T, Onouchi S, Saito S Expression patterns of prosaposin and its receptors, G protein-coupled receptor (GPR) 37 and GPR37L1 mRNAs, in the chick inner ear Cell and tissue research 2023-02-08 [PMID: 36750499]
Mohammad Rk, Sheikh FA, Ahmed N et al Cathepsin-B inhibitor CA-074 attenuates retinopathy and optic neuritis in experimental autoimmune encephalomyelitis induced in SJL/J mice Saudi Pharmaceutical Journal 2022-11-01 [PMID: 36685301] (IHC-Fr, WB, Mouse)

Details:
WB Dilutions: 1:500; IHC-Fr Dilutions: 1:300
IHC-Fr, WB Mouse
Cheng Y, Shi L, Mao H et al. The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets Frontiers in aging neuroscience 2022-06-30 [PMID: 35847680] (IHC, Primate) IHC Primate
Show All 46 Publications.

Reviews for MBP Antibody (NB600-717) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Feline.

Reviews using NB600-717:
Filter by Applications
WB
(1)
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MBP NB600-717
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 04/13/2019
View

Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord
SpeciesMouse
Lot180405
Immunohistochemistry-Frozen MBP NB600-717
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/13/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedbrain and spinal cord
SpeciesMouse
Lot180405
  4
reviewed by:
Verified Customer
IHC-Fr Feline 12/08/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedbrain and spinal cord
SpeciesFeline
Lot161031

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MBP Antibody (NB600-717) (0)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
04/13/2019
Application: WB
Species: Mouse

Verified Customer
04/13/2019
Application: IHC-Fr
Species: Mouse

Verified Customer
12/08/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol MBP
Entrez
Uniprot