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PRMT2 Antibody (OTI3A3)

Images

 
Western Blot: PRMT2 Antibody (3A3) [NBP2-01291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PRMT2 Antibody (3A3) [NBP2-01291] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRMT2.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Human Kidney tissue using anti-PRMT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRMT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRMT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRMT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PRMT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Human endometrium tissue using anti-PRMT2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.51 mg/ml

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PRMT2 Antibody (OTI3A3) Summary

Immunogen
Full length human recombinant protein of human PRMT2(NP_001526) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.51 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRMT2 Antibody (OTI3A3)

  • EC 2.1.1.-
  • EC 2.1.1.125
  • Histone-arginine N-methyltransferase PRMT2
  • HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1
  • HMT1 hnRNP methyltransferase-like 1 (S. cerevisiae)
  • HMT1 hnRNP methyltransferase-like 1
  • HMT1
  • HRMT1L1PRMT2 beta
  • MGC111373
  • PRMT2 alpha
  • PRMT2 gamma
  • protein arginine methyltransferase 2
  • protein arginine N-methyltransferase 2

Background

PRMT2 is a member of the protein arginine N-methyltransferase family that has been shown to be both an androgen receptor (AR)-associated coactivator and an estrogen receptor. PRMT2 expression is upregulated in the lung under hypoxic conditions, and has been shown to inhibit cell activation and promote programmed cell death through a NF-kappaB-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRMT2
Entrez