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PDGFR alpha Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by ...read more
PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more
PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained ...read more
Recombinant Human PDGF-AA (221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002. Proliferation elicited by Recombinant ...read more
Extracellularly applied recombinant human alpha -syn PFFs induced cytoplasmic alpha -syn-immunoreactive inclusions in primary BCAS1(+) cell cultures. Immunostaining of oligodendroglial cells incubated with 1 μM ...read more
Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the mouse VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark ...read more
Leptin promotes OPC proliferation. Representative image of cultured OPCs stained with antibodies against LepRb (green) and PDGFR alpha (red). Scale bar: 25 μm. Image collected and cropped by CiteAb from the ...read more
LPC injection does not enhance leptin expression in the CNS. Representative images of LepRb (green) expression in combination with PDGFR alpha , GFAP NeuN, and CD11b (red) in the mouse spinal cord with or without LPC ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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PDGFR alpha Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Specificity
Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha , rhPDGF R beta , and recombinant mouse PDGF R beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRA
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2 - 1.6 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 4 Reviews rated 5
using
AF1062 in the following applications:

Publications
Read Publications using
AF1062 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFR alpha Antibody [Unconjugated]

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGFR alpha Antibody (AF1062)(300)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, African Green Monkey, Transgenic Mouse, Xenograft.

We have publications tested in 16 applications: Bioassay, Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC frozen fixed, IHC-Fr, IHC-P, IHC/IF, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(6)
ICC
(16)
ICC/IF
(1)
IF
(3)
IHC
(95)
IHC frozen fixed
(1)
IHC-Fr
(8)
IHC-P
(11)
IHC/IF
(3)
Immunocytochemistry
(4)
Immunohistochemistry
(19)
Immunohistochemistry-Frozen
(1)
Immunoprecipitation
(2)
Neutralization
(1)
Western Blot
(10)
All Applications
Filter By Species
Human
(4)
Mouse
(143)
Rat
(7)
African Green Monkey
(1)
Transgenic Mouse
(17)
Xenograft
(2)
All Species
Showing Publications 1 - 10 of 300. Show All 300 Publications.
Publications using AF1062 Applications Species
Horiuchi, M;Watanabe, S;Komine, O;Takahashi, E;Kaneko, K;Itohara, S;Shimada, M;Ogi, T;Yamanaka, K; ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice Acta neuropathologica communications 2024-11-27 [PMID: 39605053] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Kyung, DS;Lee, E;Chae, S;Son, Y;Moon, YJ;Hwang, D;Kim, JK;Lee, YH;Seong, JK; Single-cell transcriptomic analysis reveals dynamic activation of cellular signaling pathways regulating beige adipogenesis Experimental & molecular medicine 2024-10-28 [PMID: 39465352] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Duncan, GJ;Ingram, SD;Emberley, K;Hill, J;Cordano, C;Abdelhak, A;McCane, M;Jenks, JE;Jabassini, N;Ananth, K;Ferrara, SJ;Stedelin, B;Sivyer, B;Aicher, SA;Scanlan, TS;Watkins, TA;Mishra, A;Nelson, JW;Green, AJ;Emery, B; Remyelination protects neurons from DLK-mediated neurodegeneration. Nature communications 2024-10-23 [PMID: 39443516] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Yoshida, S;Yoshida, T;Inukai, K;Kato, K;Yura, Y;Hattori, T;Enomoto, A;Ohashi, K;Okumura, T;Ouchi, N;Kawase, H;Wettschureck, N;Offermanns, S;Murohara, T;Takefuji, M; Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion Nature communications 2024-09-12 [PMID: 39266515] (Immunocytochemistry, Mouse, Transgenic Mouse) Immunocytochemistry Mouse, Transgenic Mouse
Fujikawa, Y;Kato, K;Unno, K;Narita, S;Okuno, Y;Sato, Y;Takefuji, M;Murohara, T; Dynamic upregulation of retinoic acid signal in the early postnatal murine heart promotes cardiomyocyte cell cycle exit and maturation Scientific reports 2024-08-30 [PMID: 39215116] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Liu, Y;Yin, M;Mao, X;Wu, S;Wei, S;Heng, S;Yang, Y;Huang, J;Guo, Z;Li, C;Ji, C;Hu, L;Liu, W;Zhang, LJ; Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics eLife 2024-08-30 [PMID: 39213029] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Jörg, LM;Schlötzer-Schrehardt, U;Lefebvre, V;Sock, E;Wegner, M; Transcription Factors Sox8 and Sox10 Contribute with Different Importance to the Maintenance of Mature Oligodendrocytes International journal of molecular sciences 2024-08-11 [PMID: 39201442] (Immunohistochemistry-Frozen, Mouse) Immunohistochemistry-Frozen Mouse
Ahuja, K;Vandenabeele, M;Nami, F;Lefevere, E;Van Hoecke, J;Bergmans, S;Claes, M;Vervliet, T;Neyrinck, K;Burg, T;De Herdt, D;Bhaskar, P;Zhu, Y;Looser, ZJ;Loncke, J;Gsell, W;Plaas, M;Agostinis, P;Swinnen, JV;Van Den Bosch, L;Bultynck, G;Saab, AS;Wolfs, E;Chai, YC;Himmelreich, U;Verfaillie, C;Moons, L;De Groef, L; A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome Acta neuropathologica communications 2024-08-28 [PMID: 39198924] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Bame, X;Hill, RA; Mitochondrial network reorganization and transient expansion during oligodendrocyte generation Nature communications 2024-08-14 [PMID: 39143079] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Rodrigues-Amorim, D;Bozzelli, PL;Kim, T;Liu, L;Gibson, O;Yang, CY;Murdock, MH;Galiana-Melendez, F;Schatz, B;Davison, A;Islam, MR;Shin Park, D;Raju, RM;Abdurrob, F;Nelson, AJ;Min Ren, J;Yang, V;Stokes, MP;Tsai, LH; Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice Nature communications 2024-08-08 [PMID: 39112447] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Show All 300 Publications.

Reviews for PDGFR alpha Antibody (AF1062) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF1062:
Filter by Applications
ICC
(2)
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry PDGFR alpha AF1062
Enlarge
5
reviewed by:
Kevin Thorburn
ICC Mouse 07/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary Oligodendrocyte Precursor Cells
SpeciesMouse
LotHMQ0217091

Comments

CommentsCells were obtained via immunopanning Foo et al. 2011 and grown in a defined, serum-free media for 3 days. Cells were fixed with 4% PFA for 10 mins, washed 3 times with D-PBS containing Ca2+ and Mg2+, blocked in D-PBS containing 10% donkey serum for 30min and stained overnight with PDGFR alpha antibody final concentration 0.4 micrograms/mL in D-PBS containing 2% donkey serum and 2% BSA. The next day, cells were washed with D-PBS three times and incubated with Alexa Fluor 488 conjugated Jackson Immuno donkey anti-goat IgG H+L for 30 mins at room temperature. Cells were then washed 3 times with D-PBS and visualized.
Immunohistochemistry-Frozen PDGFR alpha AF1062
Enlarge
5
reviewed by:
Jun Cai
IHC-Fr Mouse 03/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotHMQ0217031
Immunocytochemistry PDGFR alpha AF1062
Enlarge
5
reviewed by:
Xing Fu
ICC Mouse 08/12/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse skeletal muscle cells,mouse skeletal muscle primary cells,mouse skeletal muscle-derived cells
SpeciesMouse
LotHMQ0217111
Immunohistochemistry-Frozen PDGFR alpha AF1062
Enlarge
5
reviewed by:
Michelle Tallquist
IHC-Fr Mouse 01/07/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult heart,Adult Mouse Lung Tissue
SpeciesMouse
LotHMQ0215021

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Kevin Thorburn
07/12/2019
Application: ICC
Species: Mouse

Jun Cai
03/15/2019
Application: IHC-Fr
Species: Mouse

Xing Fu
08/12/2018
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PDGFRA
Uniprot