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FGFR1 Antibody (M19B2) - BSA Free

 (7 Publications)
 (1 Review)
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Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) ...read more
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
M19B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-1287AF350
Alexa Fluor 405
NB600-1287AF405
Alexa Fluor 488
NB600-1287AF488
Alexa Fluor 532
NB600-1287AF532
Alexa Fluor 594
NB600-1287AF594
Alexa Fluor 647
NB600-1287AF647
Alexa Fluor 700
NB600-1287AF700
Alexa Fluor 750
NB600-1287AF750
Allophycocyanin
NB600-1287APC
Biotin
NB600-1287B
CoraFluor 1
NB600-1287CL1
DyLight 350
NB600-1287UV
DyLight 405
NB600-1287V
DyLight 488
NB600-1287G
DyLight 550
NB600-1287R
DyLight 594
NB600-1287DL594
DyLight 650
NB600-1287C
DyLight 680
NB600-1287FR
DyLight 755
NB600-1287IR
FITC
NB600-1287F
HRP
NB600-1287H
Janelia Fluor 525
NB600-1287JF525
Janelia Fluor 549
NB600-1287JF549
Janelia Fluor 646
NB600-1287JF646
Janelia Fluor 669
NB600-1287JF669
PE
NB600-1287PE
PerCP
NB600-1287PCP
mFluor Violet 450 SE
NB600-1287MFV450
mFluor Violet 500 SE
NB600-1287MFV500
mFluor Violet 610 SE
NB600-1287MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB600-1287SS
Azide and BSA Free
NBP2-80735
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FGFR1 Antibody (M19B2) - BSA Free Summary

Immunogen
Recombinant human ectodomain of FGFR1b expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1.
Localization
Cell Membrane
Specificity
Reacts with both alpha and beta isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 33637089)
  • Immunohistochemistry reported in scientific literature (PMID 25182532)
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 34998203)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 1 Review rated 5
using
NB600-1287 in the following applications:
Publications
Read Publications using
NB600-1287 in the following applications:

Reactivity Notes

Use in Canine reported in scientific literature (PMID:34998203) Rabbit reactivity reported in scientific literature (PMID: 31755222)..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGFR1 Antibody (M19B2) - BSA Free

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Background

FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR1 Antibody (NB600-1287)(7)

We have publications tested in 3 confirmed species: Human, Canine, Rabbit.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
Canine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB600-1287 Applications Species
Sueyoshi Y, Niwa A, Itani Y et al. Surface modification of the cubic micro-cartilage by collagenase treatment and its efficacy in cartilage regeneration for ear tissue engineering International journal of pediatric otorhinolaryngology 2022-01-03 [PMID: 34998203] (IHC-P, Canine) IHC-P Canine
Abdalla Y, Luo M, MAkilA E et al. Effectiveness of porous silicon nanoparticle treatment at inhibiting the migration of a heterogeneous glioma cell population Journal of nanobiotechnology 2021-02-26 [PMID: 33637089] (ICC/IF, Human) ICC/IF Human
Mao Y, Liu X. Q, et al. Fibroblast growth factor-2/platelet-derived growth factor enhances atherosclerotic plaque stability. J Cell Mol Med 2020-01-01 [PMID: 31755222] (WB, Rabbit) WB Rabbit
Shih CH, Chiang TB, Wang WJ. Synergistic suppression of a disintegrin acurhagin-C in combination with AZD4547 and reparixin on terminating development for human osteosarcoma MG-63 cell Biochem. Biophys. Res. Commun. 2017-08-17 [PMID: 28823917] (FLOW, Human) FLOW Human
Schwenk BM, Hartmann H, Serdaroglu A et al. TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons EMBO J. 2016-09-12 [PMID: 27621269] (WB, Human) WB Human
Fu T, Seok S, Choi S et al. MiR-34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte FGF21 signaling and SIRT1 function. Mol Cell Biol. 2014-09-02 [PMID: 25182532]
Sasaki S, Ishida T, Toyota M et al. Interferon-alpha/beta and anti-fibroblast growth factor receptor 1 monoclonal antibody suppress hepatic cancer cells in vitro and in vivo. PLoS One. 2011-05-09 [PMID: 21573021] (FLOW, Human) FLOW Human

Review for FGFR1 Antibody (NB600-1287) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB600-1287:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Lisa Renshaw
 Flow Other 12/16/2010
View

Summary

ApplicationFlow Cytometry
Sample Testedtransfected cell line
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGFR1 Antibody (NB600-1287). (Showing 1 - 2 of 2 FAQs).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Secondary Antibodies

 

Isotype Controls

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Research Areas for FGFR1 Antibody (NB600-1287)

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Blogs on FGFR1.

Check out the latest blog posts on FGFR1.
FGFR1 - regulating cell growth and proliferation in development and disease
The vertebrate fibroblast growth factor receptor (FGFR) family is an important group of proteins involved in embryonic development and the growth and proliferation of adult cells. Mutations in FGFR proteins can lead to pathologies including bone o...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0
 
 Lisa Renshaw
12/16/2010
Application: Flow
Species: Other
read this review

Bioinformatics

Gene Symbol FGFR1
Entrez
Uniprot