![Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Analysis using the Biotin conjugate of NBP1-43350. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls were used (open histogram).](http://images.novusbio.com/fullsize/PDGF-R-alpha-Antibody-APA5-Flow-Cytometry-NBP1-43350-img0003.jpg "Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Analysis using the Biotin conjugate of NBP1-43350. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls were used (open histogram).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | APA5 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Immunogen | The immunogen for this antibody was CD140a. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | PDGFRA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8cells/test. In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope. |
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| Reviewed Applications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 03/07/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for PDGFR alpha Antibody (NBP1-43350)Find related products by research area.
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![Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis](http://images.novusbio.com/fullsize/PDGF-R-alpha-Antibody-APA5-Flow-Cytometry-NBP1-43350-img0001.jpg "Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis")
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