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NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

Images

 
Western Blot: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Detection of NHE3 [p Ser552] in human kidney lysate. Image from the standard format of this antibody.
Immunohistochemistry: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Mouse intestine, image courtesy of verified customer review. Image from the standard format of this antibody.
Simple Western: NHE3/SLC9A3 Antibody (14D5) - Azide and BSA Free [NBP2-80877] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ma-Op, Pm, RbSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, KD, KO
Clone
14D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
2 mg/ml

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NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free Summary

Immunogen
Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Modification
p Ser552
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC9A3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry reported in scientific literature (PMID 21255452)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:50-1:1000
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated reported in scientific literature (PMID 31276916)
  • Knockout Validated reported in scientific literature (PMID 31276916)
  • Simple Western 1:1000
  • Western Blot 1-5 ug/ml
Application Notes
In Western Blot, a band can be seen at approx. 85 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
No Preservative
Concentration
2 mg/ml
Purity
Protein G purified

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) - Azide and BSA Free

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3

Background

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and the small intestine, followed by the kidneys and the stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SLC9A3