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NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525]

Name: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor&#174; 525]
Brand: Novus Biologicals
SKU: NB110-81529JF525
Price: 499 USD

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Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, Ma-Op, Pm, RbSpecies Glossary
Applications	WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, KD, KO
Clone	14D5
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 525

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB110-81529AF350

Alexa Fluor 405 NB110-81529AF405

Alexa Fluor 488 NB110-81529AF488

Alexa Fluor 532 NB110-81529AF532

Alexa Fluor 594 NB110-81529AF594

Alexa Fluor 647 NB110-81529AF647

Alexa Fluor 700 NB110-81529AF700

Alexa Fluor 750 NB110-81529AF750

Biotin NB110-81529B

CoraFluor 1 NB110-81529CL1

DyLight 350 NB110-81529UV

DyLight 405 NB110-81529V

DyLight 488 NB110-81529G

DyLight 550 NB110-81529R

DyLight 594 NB110-81529DL594

DyLight 650 NB110-81529C

DyLight 680 NB110-81529FR

DyLight 755 NB110-81529IR

FITC NB110-81529F

HRP NB110-81529H

Janelia Fluor 549 NB110-81529JF549

Janelia Fluor 585 NB110-81529JF585

Janelia Fluor 635 NB110-81529JF635

Janelia Fluor 646 NB110-81529JF646

Janelia Fluor 669 NB110-81529JF669

Unconjugated NB110-81529

mFluor Violet 450 SE NB110-81529MFV450

mFluor Violet 500 SE NB110-81529MFV500

mFluor Violet 610 SE NB110-81529MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation NB110-81529

Azide and BSA Free NBP2-80877

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-81529AF350
Alexa Fluor 405	NB110-81529AF405
Alexa Fluor 488	NB110-81529AF488
Alexa Fluor 532	NB110-81529AF532
Alexa Fluor 594	NB110-81529AF594
Alexa Fluor 647	NB110-81529AF647
Alexa Fluor 700	NB110-81529AF700
Alexa Fluor 750	NB110-81529AF750
Biotin	NB110-81529B
CoraFluor 1	NB110-81529CL1
DyLight 350	NB110-81529UV
DyLight 405	NB110-81529V
DyLight 488	NB110-81529G
DyLight 550	NB110-81529R
DyLight 594	NB110-81529DL594
DyLight 650	NB110-81529C
DyLight 680	NB110-81529FR
DyLight 755	NB110-81529IR
FITC	NB110-81529F
HRP	NB110-81529H
Janelia Fluor 549	NB110-81529JF549
Janelia Fluor 585	NB110-81529JF585
Janelia Fluor 635	NB110-81529JF635
Janelia Fluor 646	NB110-81529JF646
Janelia Fluor 669	NB110-81529JF669
Unconjugated	NB110-81529
mFluor Violet 450 SE	NB110-81529MFV450
mFluor Violet 500 SE	NB110-81529MFV500
mFluor Violet 610 SE	NB110-81529MFV610

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525] Summary

Immunogen	Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Modification	p Ser552
Specificity	This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	SLC9A3
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	ELISA Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunoprecipitation Knockdown Validated Knockout Validated Simple Western Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525]

isoform 3
MGC126718
NHE3
NHE3MGC126720
SLC9A3
solute carrier family 9 (sodium/hydrogen exchanger), member 3
Solute carrier family 9 member 3

Background

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ser552] Antibody (14D ...

NB110-81529JF525

Species: Hu, Mu, Rt, Po, Ma-Op, Pm, Rb

Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, KD, KO

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Bioinformatics

Gene Symbol

SLC9A3

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525]

Species Glossary

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Product Details

Order Details

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 525]

Background

Limitations

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Publications for NHE3/SLC9A3 Antibody (NB110-81529JF525) (0)

Reviews for NHE3/SLC9A3 Antibody (NB110-81529JF525) (0)

Product General Protocols

Video Protocols

FAQs for NHE3/SLC9A3 Antibody (NB110-81529JF525) (0)

Secondary Antibodies

Isotype Controls

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