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NHERF-2 Antibody

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Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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NHERF-2 Antibody Summary

Immunogen
Peptide with sequence C-DGSAWKQDPFQ corresponding to internal region according to NP_001123484.1.
Specificity
Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC9A3R2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in human kidney lysates (calculated MW of 37.4 kDa band according to BAA33216.1). IHC-P: Human kidney shows strong membranous staining in glomerulus-.
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-2450.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NHERF-2 Antibody

  • E3KARP
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF2
  • NHE3RF2
  • NHERF2MGC104639
  • NHERF-2NHE3 kinase A regulatory protein E3KARP
  • OCTS2
  • SIP-1SRY-interacting protein 1
  • sodium/hydrogen exchanger
  • Sodium-hydrogen exchanger regulatory factor 2
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
  • Solute carrier family 9 isoform A3 regulatory factor 2
  • TKA-1SIP1
  • Tyrosine kinase activator protein 1

Background

Gene ID(s): 9351 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NHERF-2 Antibody (NB100-2450)(1)

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Bioinformatics

Gene Symbol SLC9A3R2
Entrez
Uniprot