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NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ma-Op, Pm, RbSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, KD, KO
Clone
14D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

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NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 669] Summary

Immunogen
Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Modification
p Ser552
Specificity
This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC9A3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 669]

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3

Background

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SLC9A3