NHE3/SLC9A3 [p Ser552] Antibody (14D5) [DyLight 350] Summary
Immunogen |
Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433] |
Modification |
p Ser552 |
Specificity |
This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
SLC9A3 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunoprecipitation
- Knockdown Validated
- Knockout Validated
- Simple Western
- Western Blot
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 29510506).
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50mM Sodium Borate |
Preservative |
0.05% Sodium Azide |
Purity |
Protein G purified |
Notes
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [DyLight 350]
Background
NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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