![Western Blot: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Western-Blot-NBP2-80869-img0001.jpg "Western Blot: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rb(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 10C2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 2.4 mg/ml |
| Immunogen | A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681] |
| Localization | Cytoplasmic |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NES |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Nestin (10C2) antibody is useful for Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 2.4 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Nestin Antibody (NBP2-80869)Find related products by research area.
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Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
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Nestin: Investigating the Link Between New Brain Cells and Depression Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul... Read full blog post. |
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Nestin Antibody Products in Neuronal Cancer Research Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NES |
![Immunocytochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection re](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80869-img0005.jpg "Immunocytochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection re")
![Immunohistochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - GFAP and Nestin immunoreactivity in retinal sections. Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80869-img0004.jpg "Immunohistochemistry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - GFAP and Nestin immunoreactivity in retinal sections. Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at")
![Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80869-img0007.jpg "Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 650.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80869-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80869-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute. Image from the standard format of this antibody.")
![Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80869-img0006.jpg "Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.")
![Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80869-img0008.jpg "Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.")
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