![Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SK-MEL-28 cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-DyLight-550-Flow-Cytometry-NB300-266R-img0002.jpg "Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SK-MEL-28 cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.")
| Reactivity | Hu, Mu, Rb(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 10C2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 550 |
| Immunogen | A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681] |
| Localization | Cytoplasmic |
| Marker | Neural Stem Cell Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NES |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Nestin Antibody (NB300-266R)Find related products by research area.
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Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
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Nestin: Investigating the Link Between New Brain Cells and Depression Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul... Read full blog post. |
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Nestin Antibody Products in Neuronal Cancer Research Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NES |
![Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-DyLight-550-Flow-Cytometry-NB300-266R-img0001.jpg "Flow Cytometry: Nestin Antibody (10C2) [DyLight 550] [NB300-266R] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.")
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