CRM1 Antibody

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IP, Simple Western, WB.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NB100-79802	Applications	Species
Kim, WK;Buckley, AJ;Lee, DH;Hiroto, A;Nenninger, CH;Olson, AW;Wang, J;Li, Z;Vikram, R;Adzavon, YM;Yau, TY;Bao, Y;Kahn, M;Geradts, J;Xiao, GQ;Sun, Z; Androgen deprivation induces double-null prostate cancer via aberrant nuclear export and ribosomal biogenesis through HGF and Wnt activation Nature communications 2024-02-09 [PMID: 38336745]
Wang XQD, Fan D, Han Q et al. Mutant NPM1 Hijacks Transcriptional Hubs to Maintain Pathogenic Gene Programs in Acute Myeloid Leukemia Cancer discovery 2023-03-01 [PMID: 36455589] (IP, ICC/IF) Details: Cut & Run	IP, ICC/IF
Gorostieta-Salas E, Moreno-Blas D, GerOnimo-Olvera C Et al. Enhanced Activity of Exportin-1/CRM1 in Neurons Contributes to Autophagy Dysfunction and Senescent Features in Old Mouse Brain Oxidative medicine and cellular longevity 2021-08-13 [PMID: 34434486] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Meiners A, Backer S, Hadrovic I et al. Specific inhibition of the Survivin-CRM1 interaction by peptide-modified molecular tweezers Nature communications 2021-03-08 [PMID: 33686072]
Balukoff NC, Ho JJD, Theodoridis PR et al. A translational program that suppresses metabolism to shield the genome Autophagy 2020-11-08 [PMID: 33188200] (WB, Rat)	WB	Rat
Agote-Aran A, Schmucker S, Jerabkova K et al. Spatial control of nucleoporin condensation by fragile X-related proteins EMBO J. 2020-07-24 [PMID: 32706158] (ICC/IF, Human)	ICC/IF	Human
Vercruysse T, De Bie J, et al. The Second-Generation Exportin-1 Inhibitor KPT-8602 Demonstrates Potent Activity against Acute Lymphoblastic Leukemia. Clin Cancer Res 2017-05-15 [PMID: 27780859] (Simple Western)	Simple Western
Neggers JE, Vanstreels E, Baloglu E et al. Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export Oncotarget 2016-10-18 [PMID: 27634897]
Oka M, Mura S, Yamada K et al. Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes. Elife 2016-01-07 [PMID: 26740045] (WB)	WB
Oka M, Asally M, Yasuda Y et al. The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. Mol Biol Cell 2010-06-01 [PMID: 20375145] (WB, ICC/IF, Human, Mouse)	WB, ICC/IF	Human, Mouse
Liao Y, Andronov L, Liu X et al. UBAP2L drives scaffold assembly of nuclear pore complexes at the intact nuclear envelope bioRxiv 2023-08-22 (WB, Human)	WB	Human
Garcia-Aguirre I, Alamillo-Iniesta A, Rodriguez-Perez R et al. Enhanced nuclear protein export in premature aging and rescue of the progeria phenotype by modulation of CRM1 activity Aging Cell 2019-07-15 [PMID: 31305018] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Shakhparonov MI, Antipova NV, Shender VO. Expression and Intracellular Localization of Paraoxonase 2 in Different Types of Malignancies. Acta naturae. [PMID: 30397533] (ICC/IF, Human)	ICC/IF	Human
Steyaert J, Scheveneels W, Vanneste J et al. FUS-Induced neurotoxicity in drosophila is prevented by downregulating nucleocytoplasmic transport proteins Human Molecular Genetics 2018-08-24 [PMID: 30379317]
Velez-Aguilera G, de Dios Gomez-Lopez J, Jimenez-Gutierrez GE et al. Control of nuclear beta-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function Biochim. Biophys. Acta 2017-11-21 [PMID: 29175376] (WB, Mouse)	WB	Mouse
von Knethen A, Tzieply N, Jennewein C et al. Casein-kinase-II-dependent phosphorylation of PPARgamma provokes CRM1-mediated shuttling of PPARgamma from the nucleus to the cytosol. J Cell Sci 2010-01-01 [PMID: 20026644]
Show All 16 Publications. Collapse Publications.

Array NB100-79802

• CRM1 Antibodies
• CRM1 ELISA Kits
• CRM1 Proteins