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RhoF Antibody (1D4B6) - BSA Free

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Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against human RHOF (AA: 1-84) recombinant protein. (Expected MW is 34.9 kDa)
Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against human RHOF (AA: 1-84) recombinant protein. (Expected MW is 34.9 kDa)
Flow Cytometry: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).
Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: RhoF Antibody (1D4B6) [NBP2-52553] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
1D4B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52553-0.025mg
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RhoF Antibody (1D4B6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human RhoF (AA: 1-84) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RHOF
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RhoF Antibody (1D4B6) - BSA Free

  • FLJ20247
  • ras homolog gene family, member F (in filopodia)
  • Rho family GTPase Rif
  • Rho in filopodia
  • rho-related GTP-binding protein RhoF
  • RIFARHF

Background

Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.; Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm > cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RHOF