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NCAPG2 Antibody

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Biological Strategies: Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunoprecipitation: NCAPG2 Antibody [NB100-1813] - Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

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NCAPG2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1143) of human Chromosome-Associated Protein G2 using the numbering given in entry AAH43404.1 (GeneID 54892).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCAPG2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 - 10 ug/mg of lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30143891). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1813 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCAPG2 Antibody

  • Chromosome-associated protein G2
  • FLJ20311
  • Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2
  • LUZP5
  • more than blood homolog
  • MTB
  • Non-SMC condensin II complex subunit G2
  • non-SMC condensin II complex, subunit G2

Background

CAP-G2 is a subunit of the heteropentameric condensin II complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II and proper segregation of sister chromatids. CAP-G2 is a non-SMC regulatory subunit of the condensin complex. Alternate names for CAP-G include condensin-II complex subunit G2, chromosome-associated protein G2, hCAP-G2, CAP-G2, Non-SMC condensin II complex subunit G2, leucine zipper protein 5, NCAPG2, LUZP5, MTB, and FLJ20311.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCAPG2 Antibody (NB100-1813)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NCAPG2
Entrez
Uniprot