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Embigin/EMB Antibody

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Western Blot: Embigin/EMB Antibody [NBP2-24689] - Analysis of Embigin/EMB in MCF7 lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW lysate using Embigin/EMB antibody at 0.25 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Embigin/EMB Antibody Summary

Immunogen
A portion of amino acids 130-180 of human Embigin was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EMB
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.25-2 ug/ml
Publications
Read Publications using NBP2-24689.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for Embigin/EMB Antibody

  • EMB
  • embigin homolog (mouse)
  • embigin homolog
  • Embigin
  • F9
  • GP70
  • H
  • MGC71745

Background

Embigin (EMB) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily (IgSF) proteins. It is a cell adhesion molecule that is preferentially expressed during mouse embryogenesis (1). Embigin, along with basigin and neuroplastin, form a small subgroup within the IgSF, with all members possessing a glutamate at the same position in the transmembrane domain (2). Mature, human Embigin is highly glycosylated and consists of an extracellular domain (ECD) wjth two Ig domains, a transmembrane region, and a short intracellular region. Within the ECD, human EMB shares 75% and 62% amino acid identity with mouse and rat EMB, respectively. Embigin is an accessory protein of monocarboxylic acid transporter, MCT2 which participates in transporting lactic acid between glial cells and neurons (3). Thus, it may be related to brain energy metabolism and has been investigated as a susceptible gene for schizophrenia (4). Embigin is highly expressed in early embryos of mice, and in the heart, lung, brain and other tissues of adult rats (5, 6). Loss of embigin promotes proliferation, anchorage-independent growth, and migration ability of breast cancer cells (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Embigin/EMB Antibody (NBP2-24689)(2)


Showing Publications 1 - 2 of 2.
Publications using NBP2-24689 Applications Species
Newton S, Aguilar C, Bunton-Stasyshyn R et al. Absence of Embigin accelerates hearing loss and causes sub-viability, brain and cardiac defects in C57BL/6N mice due to interaction with Cdh23 iScience 2023-10-20
Sherylanne Newton, Carlos Aguilar, Rosie K. Bunton-Stasyshyn, Marisa Flook, Michelle Stewart, Walter Marcotti, Steve Brown, Michael R. Bowl Absence of Embigin accelerates hearing loss and causes sub-viability, brain and heart defects in C57BL/6N mice due to interaction with Cdh23 ahl iScience 2023-09-26 [PMID: 37854703]

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EMB
Uniprot