Home » Chymase/CMA1/Mast Cell Chymase » Chymase/CMA1/Mast Cell Chymase Antibodies » Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]

 (3 Publications)
 (2 Reviews)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG ...read more
Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
422818
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4099-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946
Specificity
Detects human Chymase/CMA1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) MCPT-1, rmMCPT-6, rmMCPT-7, or rmMCPT-11.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CMA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
MAB4099 in the following applications:
Publications
Read Publications using
MAB4099 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]

  • Alpha-chymase
  • chymase 1 preproprotein transcript E
  • chymase 1 preproprotein transcript I
  • chymase 1, mast cell
  • Chymase
  • chymase, heart
  • chymase, mast cell
  • CMA1
  • CYH
  • CYM
  • EC 3.4.21
  • EC 3.4.21.39
  • Mast cell protease I
  • MCP3P
  • Mcpt5
  • MCT1
  • MGC119890
  • MGC119891

Background

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

  1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
  2. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
  3. Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
  4. Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MCT1/SLC16A1
Antibody - BSA Free
NBP1-59656
Western Blot MCT1/SLC16A1 Antibody - BSA Free
Western Blot MCT1/SLC16A1 Antibody - BSA Free
Western Blot MCT1/SLC16A1 Antibody - BSA Free
(15 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
ACE/CD143 Antibody
[Unconjugated]
AF1513
Immunohistochemistry ACE/CD143 Antibody [Unconjugated]
Western Blot ACE/CD143 Antibody [Unconjugated]
Simple Western ACE/CD143 Antibody [Unconjugated]
(1 Review)
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
SLC16A4 Antibody
NBP2-13315
Immunohistochemistry-Paraffin SLC16A4 Antibody
Immunohistochemistry-Paraffin SLC16A4 Antibody
Immunohistochemistry-Paraffin SLC16A4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SLC16A3 Antibody
NBP1-81251
Immunocytochemistry/ Immunofluorescence SLC16A3 Antibody
Immunohistochemistry-Paraffin SLC16A3 Antibody
Immunohistochemistry-Paraffin SLC16A3 Antibody
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
MCT2 Antibody
NB300-877
Immunohistochemistry-Paraffin MCT2 Antibody
Western Blot MCT2 Antibody
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
MCAT Antibody (2F2)
H00027349-M01
Immunohistochemistry-Paraffin MCAT Antibody (2F2)
Western Blot MCAT Antibody (2F2)
Sandwich ELISA MCAT Antibody (2F2)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
MCPH1 Antibody
AF3998
Western Blot MCPH1 Antibody
(1 Review)
(4 Publications)
Species: Hu
Applications: WB
SCF/c-kit Ligand
[Unconjugated]
255-SC
SCF/c-kit Ligand [Unconjugated]
(202 Publications)
Species: Hu
Applications: BA
Angiogenin [HRP]
DAN00
N/A Angiogenin [HRP]
N/A Angiogenin [HRP]
(23 Publications)
Species: Hu
Applications: ELISA
Cathepsin G
Antibody
NBP2-33498
Immunohistochemistry-Paraffin Cathepsin G Antibody
Immunohistochemistry-Paraffin Cathepsin G Antibody
Immunohistochemistry-Paraffin Cathepsin G Antibody
(5 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western Blot EMMPRIN/CD147 Antibody [Unconjugated]
Immunohistochemistry EMMPRIN/CD147 Antibody [Unconjugated]
Knockout Validated EMMPRIN/CD147 Antibody [Unconjugated]
(1 Review)
(15 Publications)
Species: Hu
Applications: ELISA, IHC, KO, Simple Western, WB
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western CD117/c-kit Antibody [Unconjugated]
Western Blot CD117/c-kit Antibody [Unconjugated]
Immunohistochemistry CD117/c-kit Antibody [Unconjugated]
(4 Reviews)
(116 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Simple Western, WB
Glut1 Antibody -
BSA Free
NB110-39113
Western Blot Glut1 Antibody - BSA Free
Flow Cytometry Glut1 Antibody - BSA Free
Western Blot Glut1 Antibody - BSA Free
(7 Reviews)
(62 Publications)
Species: Hu, Mu, Rb, Rt
Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB
Renin Antibody
(411507) - Propeptide
MAB4447
Immunohistochemistry Renin Antibody (411507) - Propeptide
(6 Publications)
Species: Hu
Applications: IHC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
Tryptase
gamma-1/TPSG1 Antibod ...
NBP2-01679
Flow Cytometry Tryptase gamma-1/TPSG1 Antibody (OTI1G1)
Flow Cytometry Tryptase gamma-1/TPSG1 Antibody (OTI1G1)
Western Blot Tryptase gamma-1/TPSG1 Antibody (OTI1G1)
Species: Hu
Applications: Flow, IHC, IHC-P, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA
Neutrophil
Elastase/ELA2 [Biotin
DY4517-05
N/A Neutrophil Elastase/ELA2 [Biotin]
(15 Publications)
Species: Mu
Applications: ELISA
Chymase/CMA1/Mast
Cell Chymase Antibody ...
MAB4099
Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]
Species: Hu
Applications: WB, ELISA, IP

Publications for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Rat
(1)
All Species

Reviews for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB4099:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22180785
SpeciesHuman
  5
reviewed by:
Verified Customer
 WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23312967
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Chymase/CMA1/Mast Cell Chymase Products

Blogs on Chymase/CMA1/Mast Cell Chymase

There are no specific blogs for Chymase/CMA1/Mast Cell Chymase, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0
 
 Verified Customer
02/20/2015
Application: WB
Species: Human
read this review
 
 Verified Customer
02/20/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol CMA1
Uniprot