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Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

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Western Blot: Tryptase gamma Antibody (1G1) [NBP2-01679] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tryptase gamma (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tryptase gamma.
Western Blot: Tryptase gamma Antibody (1G1) [NBP2-01679] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tryptase gamma (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunohistochemistry-Paraffin: Tryptase gamma Antibody (1G1) [NBP2-01679] Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Tryptase gamma mouse monoclonal antibody.
Flow Cytometry: Tryptase gamma Antibody (1G1) [NBP2-01679] - Analysis of Jurkat cells, using anti-Tryptase gamma antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: Tryptase gamma Antibody (1G1) [NBP2-01679] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Tryptase gamma mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tryptase gamma Antibody (1G1) [NBP2-01679] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Tryptase gamma mouse monoclonal antibody.
Flow Cytometry: Tryptase gamma Antibody (1G1) [NBP2-01679] - Analysis of Hela cells, using anti-Tryptase gamma antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74652AF350
Alexa Fluor 405
NBP2-74652AF405
Alexa Fluor 488
NBP2-74652AF488
Alexa Fluor 532
NBP2-74652AF532
Alexa Fluor 594
NBP2-74652AF594
Alexa Fluor 647
NBP2-74652AF647
Alexa Fluor 700
NBP2-74652AF700
Alexa Fluor 750
NBP2-74652AF750
Allophycocyanin
NBP2-74652APC
Allophycocyanin/Cy7
NBP2-74652APCCY7
Biotin
NBP2-74652B
CoraFluor 1
NBP2-74652CL1
DyLight 350
NBP2-74652UV
DyLight 405
NBP2-74652V
DyLight 488
NBP2-74652G
DyLight 550
NBP2-74652R
DyLight 594
NBP2-74652DL594
DyLight 650
NBP2-74652C
DyLight 680
NBP2-74652FR
DyLight 755
NBP2-74652IR
FITC
NBP2-74652F
HRP
NBP2-74652H
Janelia Fluor 525
NBP2-74652JF525
Janelia Fluor 549
NBP2-74652JF549
Janelia Fluor 585
NBP2-74652JF585
Janelia Fluor 635
NBP2-74652JF635
Janelia Fluor 646
NBP2-74652JF646
Janelia Fluor 669
NBP2-74652JF669
PE
NBP2-74652PE
PE/Atto594
NBP2-74652PEATT594
PE/Cy5.5
NBP2-74652PECY55
PE/Cy7
NBP2-74652PECY7
PerCP
NBP2-74652PCP
mFluor Violet 450 SE
NBP2-74652MFV450
mFluor Violet 500 SE
NBP2-74652MFV500
mFluor Violet 610 SE
NBP2-74652MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74652
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Tryptase gamma-1/TPSG1 Antibody (OTI1G1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 20-283 of human TPSG1(NP_036599) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPSG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

  • EC 3.4.21
  • EC 3.4.21.-
  • gamma I
  • gamma II
  • lung tryptase
  • mast cell protease II
  • mast cell tryptase
  • PRSS31skin tryptase
  • Serine protease 31
  • TMTpituitary tryptase
  • TPSG1
  • Transmembrane tryptase
  • trpA
  • tryptase gamma 1
  • tryptase gamma I
  • tryptase gamma II
  • tryptase gamma
  • Tryptase gamma1
  • Tryptase gamma-1

Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-01679
Tryptase gamma-1/TPSG1 Antibody (OTI1G1)
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Bioinformatics

Gene Symbol TPSG1
Entrez