Nestin Antibodies
We offer Nestin Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Western Blot. Each Nestin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Nestin Antibodies can be used in a variety of model species: Canine, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the Nestin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Nestin polyclonal antibodies and browse our Nestin monoclonal antibody catalog.
Alternate Names for Nestin Antibodies
anti-Nestin antibody, anti-NES antibody, anti-FLJ21841 antibody
12 Results for "nestin" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) Scale bar = 20 um. NCSC = neural crest stem cell, MSC = mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1604-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. NCSCmix express Nestin (green), Sox2 (red) Scale bar = 20 um. NCSC = neural crest stem cell, MSC = mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB100-1604] - Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. MSCmix are adherent fibroblast-like cells, do not express Sox2 c (red), slightly express Nestin (c) (green). Scale bar = 20 um. MSC mesenchymal stem cell. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/211) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1604-img0007.jpg)
![Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Frozen-NB100-1604-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - In vitro proliferation & differentiation of ahMNCs-Clump II. (A) ahMNCs-Clump II were propagated in the culture condition for ahMNCs. The accumulated number of cells of ahMNCs-Clump II (NS18-007TL & 008TL) in comparison with ahMNCs established using previous culture methods (NS14-010TL, NS14-011TL, & NS15-001TL). Days to 1 × 109 cells are indicated. (B) Morphologies of ahMNCs-Clump II under expansion processes are illustrated until third in vitro passage (P3). Scale bar = 50 μm. (C) After in vitro differentiation, immunofluorescence was applied to ahMNCs-Clump II. Nestin for NSCs, MAP2 & Tuj1 for neurons, & GFAP for astrocytes. Undiffer = before in vitro differentiation; Differ = after in vitro differentiation. Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30380605), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-310202415291944.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Representative images of nestin (A) & Tmsb4x (B) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (C) Merged images showing Tmsb4x (red) & nestin (green) staining; areas of colocalization are indicated by arrows. Representative images of Tmsb4x (D) & Cd31 (E) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (F) Merged images showing Tmsb4x (green) & Cd31 (red) staining. Bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27575556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-310202415304248.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Dopaminergic neuron derivation from H9 stem cells & hIPSCs.Scheme showing the different steps of the DA neuron derivation protocol (A). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) for different NSCs markers, Sox1, Pax6 & Nestin (B). Immunolabeling of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 3 days of DA progenitor induction for progenitor markers Lmx1A & FoxA2 (C) & after 7 days of induction for immature neurons beta III-Tubulin+ & remaining progenitors Sox1+ (D). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 21 days of induction for the neuronal marqueur MAP2 & DA neurons Tyrosine Hydroxylase (TH) (E). Analysis of gene expression levels by qRT-PCR for cultured H9 (top panels) & WT hiPSCs at different time points (NSC, 14 days & 21 days) of the culture (FB = fetal brain) (F,G). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241555236.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Neural Stem Cell derivation from WT & LRRK2 G2019S hiPSCs.Haematoxylin & eosin coloration of teratoma-encapsulated tumors generated by flank injection of WT & G2019S hiPSCs cells in Nod/Scid mice. Representative images of the different germ layers: ectoderm (immature squamous epithelium, neural rosettes), mesoderm (primitive cartilage, muscles, fat) & endoderm (primitive gut like epithelium) (A). Scheme showing the different steps of the DA neuron derivation protocol (B). Immunolabelings of cultured WT & LRRK2 G2019S hIPSCs for different NSCs markers after NSCs induction protocol: Sox1, Nestin, Pax6 & Ki67 (C). Analysis of gene expression levels by qRT-PCR for WT & LRRK2 G2019S cultures at the end of the NSCs induction protocol (D). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241554536.jpg)
![Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. Photomicrographs show immunofluorescence double-labeling for PAR1 with Sox2-positive (A), or PAR1 with Nestin-positive (C) multipotent neural stem cells (NSCs) within the lateral wall of the lateral ventricle (LV). RNAscope was used to identify cells expressing both PAR1 & Sox2 (B), or Nestin (D) RNA in NSCs of the adult SVZ. Arrow indicates an example of a double-labeled cell in each case, with arrowhead indicating a singly labeled cell (Scale bar = 10 μm). Boxed area in B & D is also shown at higher magnification to visualize double-labeled cells. (E) Histogram shows expression of PAR1 RNA was high in NSCs grown as neurospheres (NS), or when plated on poly-L-lysine coated coverslips as monolayers in stem cell media containing EGF & bFGF. PAR1 RNA expression by NSC monolayers decreased by 87% when EGF & bFGF were removed from the media for 7 DIV promoting stem cell differentiation. (F) Withdrawal of EGF & bFGF to induce NSC monolayer differentiation resulted in a parallel decrease in Nestin RNA expression. (**P < 0.01, ***P < 0.001 Students t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29921916), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1604_chicken-polyclonal-nestin-antibody-31020241612687.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-NB300-266-img0020.jpg "Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.")
![Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Western-Blot-NB300-266-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0015.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-Paraffin-NB300-266-img0013.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0006.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0017.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0016.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth and vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun-eosin (median panel) or with nestin antibody (upper and lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, and each surface was measured in mm^2. The red rectangles indicate the zone of the tumour shown in median (hemalun-eosin staining) and lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median and lower panel 100 um. The graph shows the tumour size in mm^2 (expressed as the mean +/- SEM of 4 mice). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-20920231412574.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth & vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. (A) Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun–eosin (median panel) or with nestin antibody (upper & lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, & each surface was measured in mm2. The red rectangles indicate the zone of the tumour shown in median (hemalun–eosin staining) & lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median & lower panel 100 µm. The graph shows the tumour size in mm2 (expressed as the mean ± SEM of 4 mice). (B) Representative immunohistochemistry photographs of CD31+ vessels in brain tumour areas of 3 different mice treated with regorafenib (right panel) or without regorafenib (control, left panel). Arrows indicate CD31+ blood vessels. Scale bars, 20 µm. The graph shows the number of CD31+ vessels in tumours per mm2 (expressed as mean ± SEM of 4 mice). * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-310202416212379.jpg)
![Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Western-Blot-NBP1-92717-img0006.jpg "Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0005.jpg)
![Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunohistochemistry-NBP1-92717-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for Vimentin and so appear red. Others contain Vimentin and not Nestin and so appear green - these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0004.jpg)
![Western Blot: Nestin Antibody [NB300-265] - Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug). Lane 1: 0.5 ug/ml NB 300-265: lane 2: 2 ug/ml NB 300-265. ECL: 1 second exposure.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NB300-265-img0002.jpg "Western Blot: Nestin Antibody [NB300-265] - Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug). Lane 1: 0.5 ug/ml NB 300-265: lane 2: 2 ug/ml NB 300-265. ECL: 1 second exposure.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB300-265] - The Nestin antibody was tested in NTERA2 cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-265-img0003.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48504-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0014.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48504 (A) shows similar protein distribution across tissues to independent antibody NBP2-48575 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0010.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0013.jpg)
![Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0004.jpg "Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775] - Staining of RH-30 cells using the Anti-NES monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunocytochemistry-Immunofluorescence-NBP2-59775-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-NES monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0003.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58884-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.")