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Nestin Antibody (196908) [Phycoerythrin]

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NTera-2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 µM Retinoic Acid for 6 days (light orange filled histogram) was stained with Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
196908
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

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Nestin Antibody (196908) [Phycoerythrin] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Specificity
Detects human Nestin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC1259P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908) [Phycoerythrin]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (IC1259P)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, ICC.


Filter By Application
Flow Cytometry
(8)
ICC
(1)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using IC1259P Applications Species
A Longoni, L Utomo, IE van Hooijd, GK Bittermann, VC Vetter, EC Kruijt Spa, J Ross, AJ Rosenberg, D Gawlitta The chondrogenic differentiation potential of dental pulp stem cells Eur Cell Mater, 2020-02-21;39(0):121-135. 2020-02-21 [PMID: 32083715] (Flow Cytometry, Human) Flow Cytometry Human
H Yoshida, L Koodie, K Jacobsen, K Hanzawa, Y Miyamoto, M Yamamoto B4GALNT1 induces angiogenesis, anchorage independence growth and motility, and promotes tumorigenesis in melanoma by induction of ganglioside GM2/GD2 Sci Rep, 2020-01-27;10(1):1199. 2020-01-27 [PMID: 31988291] (Flow Cytometry, Human) Flow Cytometry Human
AA Delbandi, M Mahmoudi, A Shervin, S Heidari, R Kolahdouz-, AH Zarnani Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls BMC Womens Health, 2020-01-06;20(1):3. 2020-01-06 [PMID: 31906916] (Flow Cytometry, Human) Flow Cytometry Human
Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Frontiers in Bioengineering and Biotechnology, 2018-11-01;0(0):2296-4185. 2018-11-01 [PMID: 30443545] (ICC, Human) ICC Human
MA Avanzini, V Abbonante, P Catarsi, I Dambruoso, M Mantelli, V Poletto, E Lenta, P Guglielmel, S Croce, L Cobianchi, B Jemos, R Campanelli, E Bonetti, CA Di Buduo, S Salmoiragh, L Villani, M Massa, M Boni, R Zappatore, A Iurlo, A Rambaldi, AM Vannucchi, P Bernasconi, A Balduini, G Barosi, V Rosti The spleen of patients with myelofibrosis harbors defective mesenchymal stromal cells Am. J. Hematol., 2018-02-08;0(0):. 2018-02-08 [PMID: 29359451] (Flow Cytometry, Human) Flow Cytometry Human
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-04-25;8(17):28074-28092. 2017-04-25 [PMID: 28427209] (Flow Cytometry, Human) Flow Cytometry Human
Hasmim Meriem, Bruno Stefania, Azzi Sandy et al. Isolation and characterization of renal cancer stem cells from patient-derived xenografts. Oncotarget 1/1/2016 [PMID: 26551931]
Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M Phenotypic diversity of patient-derived melanoma populations in stem cell medium. Lab Invest, 2015-04-13;95(6):672-83. 2015-04-13 [PMID: 25867763] (Flow Cytometry, Human) Flow Cytometry Human
Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. J. Invest. Dermatol., 2010-09-30;131(2):487-94. 2010-09-30 [PMID: 20882037] (Flow Cytometry, Human) Flow Cytometry Human
Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G MDR1 expression identifies human melanoma stem cells. Biochem. Biophys. Res. Commun., 2008-02-13;368(4):930-6. 2008-02-13 [PMID: 18279661] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

Isotype Controls

Additional Nestin Products

Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

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Bioinformatics

Gene Symbol NES