![Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Western Blot Analysis of COLO38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (M2-7C10 + M2-9E3 + T311 + HMB45).](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Western-Blot-NBP2-34337-img0006.jpg "Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Western Blot Analysis of COLO38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (M2-7C10 + M2-9E3 + T311 + HMB45).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | M2-7C10 + M2-9E3 + T311 + HMB45 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34681) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. |
| Isotype | IgG2b Lambda/IgG2a Kappa/IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-NBP2-34337-img0005.jpg "Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.")
![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (M2-7C10 + M2-9E3 + T311 + HMB45).](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Paraffin-NBP2-34337-img0001.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (M2-7C10 + M2-9E3 + T311 + HMB45).")
![Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Frozen human melanoma stained for melanoma cancer cells (red) and counter-stained with DAPI (blue). This image was submitted via customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Frozen-NBP2-34337-img0002.jpg "Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Frozen human melanoma stained for melanoma cancer cells (red) and counter-stained with DAPI (blue). This image was submitted via customer review. Image from the Alexa Fluor 700 version of this antibody.")
![Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Melanoma cells (in green) stained for Mar-1 + Tyrosinase + p100. Counter stained with DAPI ( blue). Image from a customer review using Alexa Fluor 647 version of this antibody.](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Frozen-NBP2-34337-img0003.jpg "Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Melanoma cells (in green) stained for Mar-1 + Tyrosinase + p100. Counter stained with DAPI ( blue). Image from a customer review using Alexa Fluor 647 version of this antibody.")
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