![Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) - IHC-Prediluted [NBP2-48257] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-IHC-Prediluted-Immunohistochemistry-NBP2-48257-img0002.jpg "Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) - IHC-Prediluted [NBP2-48257] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | M2-7C10 + M2-9E3 + T311 + HMB45 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. |
| Isotype | IgG2b Lambda/IgG2a Kappa/IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MLANA |
![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) - IHC-Prediluted [NBP2-48257] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (M2-7C10 + M2-9E3 + T311 + HMB45).](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48257-img0001.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) - IHC-Prediluted [NBP2-48257] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (M2-7C10 + M2-9E3 + T311 + HMB45).")
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