![Western Blot: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa(A) (0.01ug/ml) cells lysate and HeLa(B) (0.01ug/ml) cells nuclear lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 21B4 (NBP3-20167) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20167_rabbit-magea3-mab-21b4-chimeric-azide-and-bsa-free-2742023924279.png "Western Blot: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa(A) (0.01ug/ml) cells lysate and HeLa(B) (0.01ug/ml) cells nuclear lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 21B4 (NBP3-20167) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | 21B4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3. |
| Specificity | This antibody binds human MAGEA3 and MAGEA6, but does not cross react with other members of the MAGE family like MAGE-A1, MAGE-A4 and MAGE-A10. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAGEA3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MAGEA3 Antibody (NBP3-20167)Find related products by research area.
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| Gene Symbol | MAGEA3 |
![Immunocytochemistry/Immunofluorescence: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21B4 (NBP3-20167) (1:1000 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20167, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20167_rabbit-magea3-mab-21b4-chimeric-azide-and-bsa-free-2742023924215.png "Immunocytochemistry/Immunofluorescence: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21B4 (NBP3-20167) (1:1000 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20167, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa cells were stained with an isotype control (NBP2-52638, black line) or the rabbit-chimeric version of 21B4 (NBP3-20167, blue line) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488(R) conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20167_rabbit-magea3-mab-21b4-chimeric-azide-and-bsa-free-2742023924213.png "Flow Cytometry: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa cells were stained with an isotype control (NBP2-52638, black line) or the rabbit-chimeric version of 21B4 (NBP3-20167, blue line) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488(R) conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")