I would like to know where I can expect to see the signals with NB100-2736 in WB. After checking the information from UniProt, the MW of MDM2 seems to be 55 KDa. However, it may be changed by phosphorylation and Ubl conjugation. Is it the reason why the image on the product page showing the band at 100 KDa?

Name: MDM2/HDM2 Antibody (SMP14) - BSA Free
Brand: Novus Biologicals
SKU: NB100-2736
Rating: 4 (1 reviews)

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This product is a bit tricky as the protein is especially prone to forming protein complexes with other proteins; specifically 53/TP53, TP73/p73, RBL5 and RP11. Due to these interactions, it is common for this protein to be detected at a much higher weight than the expected weight given in Uniprot. In our testing, this protein usually detects a band at 100kDa. It is also possible to detect lower bands at 55 and 30kDa.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, IHC, IP
Clone	SMP14
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-2736AF350

Alexa Fluor 405 NB100-2736AF405

Alexa Fluor 488 NB100-2736AF488

Alexa Fluor 532 NB100-2736AF532

Alexa Fluor 594 NB100-2736AF594

Alexa Fluor 647 NB100-2736AF647

Alexa Fluor 700 NB100-2736AF700

Alexa Fluor 750 NB100-2736AF750

Biotin NB100-2736B

CoraFluor 1 NB100-2736CL1

DyLight 350 NB100-2736UV

DyLight 405 NB100-2736V

DyLight 488 NB100-2736G

DyLight 550 NB100-2736R

DyLight 594 NB100-2736DL594

DyLight 650 NB100-2736C

DyLight 680 NB100-2736FR

DyLight 755 NB100-2736IR

FITC NB100-2736F

HRP NB100-2736H

Janelia Fluor 525 NB100-2736JF525

Janelia Fluor 549 NB100-2736JF549

Janelia Fluor 646 NB100-2736JF646

Janelia Fluor 669 NB100-2736JF669

mFluor Violet 450 SE NB100-2736MFV450

mFluor Violet 500 SE NB100-2736MFV500

mFluor Violet 610 SE NB100-2736MFV610

Catalog# & Formulation	Size	Price
BSA Free NB100-2736SS

Azide and BSA Free NBP2-80845

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2736AF350
Alexa Fluor 405	NB100-2736AF405
Alexa Fluor 488	NB100-2736AF488
Alexa Fluor 532	NB100-2736AF532
Alexa Fluor 594	NB100-2736AF594
Alexa Fluor 647	NB100-2736AF647
Alexa Fluor 700	NB100-2736AF700
Alexa Fluor 750	NB100-2736AF750
Biotin	NB100-2736B
CoraFluor 1	NB100-2736CL1
DyLight 350	NB100-2736UV
DyLight 405	NB100-2736V
DyLight 488	NB100-2736G
DyLight 550	NB100-2736R
DyLight 594	NB100-2736DL594
DyLight 650	NB100-2736C
DyLight 680	NB100-2736FR
DyLight 755	NB100-2736IR
FITC	NB100-2736F
HRP	NB100-2736H
Janelia Fluor 525	NB100-2736JF525
Janelia Fluor 549	NB100-2736JF549
Janelia Fluor 646	NB100-2736JF646
Janelia Fluor 669	NB100-2736JF669
mFluor Violet 450 SE	NB100-2736MFV450
mFluor Violet 500 SE	NB100-2736MFV500
mFluor Violet 610 SE	NB100-2736MFV610

MDM2/HDM2 Antibody (SMP14) - BSA Free

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Publications for MDM2/HDM2 Antibody (NB100-2736)(22)

Review for MDM2/HDM2 Antibody (NB100-2736) (1) 41

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Immunogen	Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
Epitope	Amino acids 154 - 167.
Localization	Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
Specificity	The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	MDM2
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Publications using NB100-2736	Applications	Species
Lee S, Jeong H, Hwang B et al. Helicobacter pylori promotes epithelial-to-mesenchymal transition by downregulating CK2B in gastric cancer cells Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2022-10-01 [PMID: 36404440]
Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)	WB	Human
Oh MS, Lee SG, Lee GH et al. In vivo tracking of 14 C thymidine labeled mesenchymal stem cells using ultra-sensitive accelerator mass spectrometry. Scientific reports 2021-08-09 [PMID: 33446731]
Kaczorowski A, Tolstov Y, Falkenstein M et al. Rearranged ERG confers robustness to prostate cancer cells by subverting the function of p53 Urol. Oncol. 2020-07-13 [PMID: 32674955] (ICC/IF, Human)	ICC/IF	Human
Breton Y, Desrosiers V, Ouellet M et al. Expression of MDM2 in macrophages promotes the early post-entry steps of HIV-1 infection through inhibition of p53. J. Virol. 2019-01-23 [PMID: 30674627] (WB, Human)	WB	Human
Ramos GV, Cruz A, Silva WJ et al. Thyroid hormone upregulates MDM2 in rat type i fiber: implications for skeletal muscle mass regulation Acta Physiol (Oxf). 2017-11-27 [PMID: 29178319] (Rat)		Rat
Joshi Y, Soria MG, Quadrato G et al. The MDM4/MDM2-p53-IGF1 axis controls axonal regeneration, sprouting and functional recovery after CNS injury. Brain. 2015-05-16 [PMID: 25981963] (WB, IHC-Fr, Mouse)	WB, IHC-Fr	Mouse
Inaba H, Kuboniwa M, Sugita H et al. Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts Infect Immun 2012-08-01 [PMID: 22689813] (WB, Human)	WB	Human
de Freitas Mda C, Ramalho LM, Xavier FC et al. p53 and MDM2 protein expression in actinic cheilitis. J Appl Oral Sci. 2008-11-01 [PMID: 19082401] (IF/IHC, Human)	IF/IHC	Human
Chen D, Zhang Z, Li M, Wang W et al. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene. 2007-08-02 [PMID: 17310983] (WB, Human)	WB	Human
Stevenson LF, Sparks A, Allende-Vega N et al. The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2. EMBO J. 2007-02-21 [PMID: 17290220] (IP, Human)	IP	Human
Paajarvi G, Roudier E, Crisby M et al. HMG-CoA reductase inhibitors, statins, induce phosphorylation of Mdm2 and attenuate the p53 response to DNA damage. FASEB J. 2005-03-01 [PMID: 15625077] (IF/IHC, Rat)	IF/IHC	Rat
Minsky N, Oren M . The RING domain of Mdm2 mediates histone ubiquitylation and transcriptional repression. Mol Cell. 2004-11-19 [PMID: 15546622] (IP, WB, Human)	IP, WB	Human
Menendez S, Higgins M, Berkson RG et al. Nuclear export inhibitor leptomycin B induces the appearance of novel forms of human Mdm2 protein. Br J Cancer. 2003-02-24 [PMID: 12592381] (WB, Human)	WB	Human
Goldberg Z, Vogt Sionov R,Berger M. Tyrosine phosphorylation of Mdm2 by c-Abl: implications for p53 regulation. EMBO J. 2002-07-15 [PMID: 12110584] (WB, Human)	WB	Human
Kersemaekers AM, Mayer f, Molier M et al. Role of P53 and MDM2 in treatment response of human germ cell tumors. J Clin Oncol. 2002-03-15 [PMID: 11896104] (IHC-P, Human)	IHC-P	Human
Hjerrild M, Milne D, Dumaz N et al. Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells. Bioche J. 2001-04-15 [PMID: 11284721] (WB, Human)	WB	Human
Rodriquez-Lopez AM, Xenaki D, Eden TO et al MDM2 mediated nuclear exclusion of p53 attenuates EPE-induced apoptosis in neuroblastoma cells Mol Pharmacol 2001-01-01 [PMID: 11125034] (WB, Human)	WB	Human
Berger M, Vogt Sionov R, Levine AJ, Haupt Y. A role for the polyproline domain of p53 in its regulation by Mdm2. J Biol Chem. 2001-02-09 [PMID: 11053443] (WB, Human)	WB	Human
Sionov RV, Moallem E, Berger M et al. c-Abl neutralizes the inhibitory effect of Mdm2 on p53. J Biol Chem 1999-03-26 [PMID: 10085066] (WB, Human)	WB	Human
Shieh SY, Ikeda M, Taya Y, Prives C. DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 1997-10-31 [PMID: 9363941] (WB, IP, Human)	WB, IP	Human
Martin K, Trouche D, Hagemeier C et al. Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature;375(6533):691-4. 1995-06-22 [PMID: 7791903] (WB)	WB
Show All 22 Publications. Collapse Publications.

	Verified Customer 09/08/2010
Application:	WB
Species:	Human

Gene Symbol	MDM2
Entrez	4193(Human)
Uniprot	Q00987()