![Western Blot: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Azide-and-BSA-Free-Western-Blot-NBP2-80845-img0001.jpg "Western Blot: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP |
| Clone | SMP14 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987] |
| Epitope | Amino acids 154 - 167. |
| Localization | Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MDM2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody has also been reported useful in IHC on resin tissue sections. Staining of IHC-P tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MDM2/HDM2 Antibody (NBP2-80845)Find related products by research area.
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| Gene Symbol | MDM2 |
![Immunohistochemistry: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2. Image from the standard format of th](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80845-img0002.jpg "Immunohistochemistry: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2. Image from the standard format of th")
![Immunohistochemistry: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2. Image from the standard format of th](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80845-img0003.jpg "Immunohistochemistry: MDM2/HDM2 Antibody (SMP14) - Azide and BSA Free [NBP2-80845] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2. Image from the standard format of th")
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