![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0005.jpg "Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF. Amino Acid Squence: GGG LRPGDCEVCISY |
| Specificity | This antibody does not cross-react with CDNF. |
| Predicted Species | Bovine (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MANF |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MANF Antibody (NBP1-76837)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: MANF Antibody [NBP1-76837] - Immunofluorescence of MANF in Human Brain tissue with MANF antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76837-img0003.jpg "Immunocytochemistry/Immunofluorescence: MANF Antibody [NBP1-76837] - Immunofluorescence of MANF in Human Brain tissue with MANF antibody at 20 ug/mL.")
![Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76837] - Human brain tissue with ARMET antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunohistochemistry-Paraffin-NBP1-76837-img0002.jpg "Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76837] - Human brain tissue with ARMET antibody at 2.5 ug/ml.")
![Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0001.jpg "Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.")
![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0004.jpg "Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
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![Knockdown Validated COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]](https://images.novusbio.com/images/pp-h7147-00_human-coup-tf-ii-nr2f2-mab-clone-h7147-41202412505337.jpg)
![Immunohistochemistry PITX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/PITX2_AF7388_Immunohistochemistry_12162.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")