Name: COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]
Brand: Novus Biologicals
SKU: PP-H7147-00
Rating: 4.3 (3 reviews)

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Date

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Immunohistochemistry-Frozen COUP-TF II/NR2F2 PP-H7147-00

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IHC-Fr

Mouse

05/13/2019

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Summary

Application	Immunohistochemistry-Frozen
Sample Tested	E17 coronal brain section
Species	Mouse
Lot	A-2

Comments

Brain sections were antigen retrieved with 10mM sodium citrate (pH6) buffer and then blocked with 10% donkey serum in PBST. primary and secondary antibodies were prepared in the same blocking buffer.

IHC-P

Rat

02/25/2015

Application	Immunohistochemistry-Paraffin
Sample Tested	See PMID 22253897
Species	Rat

Application	Immunofluorescence
Sample Tested	See PMID 22615892
Species	Rat

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC, IP
Clone	H7147
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Suitable Sample Type	23015

Immunogen	Recombinant human COUP-TF II/NR2F2 Amino Acids 43-64
Specificity	This antibody specifically recognizes human COUP-TF II and cross-reacts with rat COUP-TF II. This antibody does not cross-react with human COUP-TF I or EAR2. Not yet tested in other species.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	NR2F2
Purity Statement	Protein A or G purified from ascites
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer	A liquid formulation in physiologic saline with 0.1% NaN₃.
Preservative	0.1% Sodium Azide

Publications using PP-H7147-00	Applications	Species
Li, J;Wiesinger, A;Fokkert, L;Bakker, P;de Vries, DK;Tijsen, AJ;Pinto, YM;Verkerk, AO;Christoffels, VM;Boink, GJJ;Devalla, HD; Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids Cell stem cell 2024-09-05 [PMID: 39260368] (Immunocytochemistry, Human)	Immunocytochemistry	Human
ES Liau, S Jin, YC Chen, WS Liu, M Calon, S Nedelec, Q Nie, JA Chen Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons Oncogene, 2023-01-03;14(1):46. 2023-01-03 [PMID: 36596814]
J Li, A Wiesinger, L Fokkert, BJ Boukens, AO Verkerk, VM Christoffe, GJJ Boink, HD Devalla Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac models Journal of tissue engineering, 2022-10-18;13(0):2041731422112. 2022-10-18 [PMID: 36277058] (ICC, Human)	ICC	Human
D Yang, J Gomez-Garc, S Funakoshi, T Tran, I Fernandes, GD Bader, MA Laflamme, GM Keller Modeling human multi-lineage heart field development with pluripotent stem cells Cell Stem Cell, 2022-09-01;29(9):1382-1401.e8. 2022-09-01 [PMID: 36055193] (ICC, Human)	ICC	Human
KF Rodriguez, PR Brown, CM Amato, B Nicol, CF Liu, X Xu, HH Yao Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B Nature Communications, 2022-07-15;13(1):4130. 2022-07-15 [PMID: 35840551] (IHC, Mouse)	IHC	Mouse
Wei Feng, Hannah Schriever, Shan Jiang, Abha Bais, Haodi Wu, Dennis Kostka et al. Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency Communications Biology 2022-04-29 [PMID: 35488063]
X Zhu, Y Guo, C Chu, D Liu, K Duan, Y Yin, C Si, Y Kang, J Yao, X Du, J Li, S Zhao, Z Ai, Q Zhu, W Ji, Y Niu, T Li BRN2 as a key gene drives the early primate telencephalon development Science Advances, 2022-03-04;8(9):eabl7263. 2022-03-04 [PMID: 35245119] (IHC, Primate (Macaca fascicularis))	IHC	Primate (Macaca fascicularis)
Feng Liu, Dandan Long, Wenjun Huang, Wanling Peng, Huan Lan, Yafei Zhou et al. The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSC International Journal of Stem Cells 2022-08-30 [PMID: 35220280]
Barroso-GonzAlez J, GarcIa-ExpOsito L, Galaviz P et al. Anti-recombination function of MutS alpha restricts telomere extension by ALT-associated homology-directed repair Cell reports 2021-12-07 [PMID: 34879271]
K Sasaki, A Oguchi, K Cheng, Y Murakawa, I Okamoto, H Ohta, Y Yabuta, C Iwatani, H Tsuchiya, T Yamamoto, Y Seita, M Saitou The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell Reports, 2021-05-04;35(5):109075. 2021-05-04 [PMID: 33951437] (IHC, Cynomolgus Monkey)	IHC	Cynomolgus Monkey
Fei Zhao, Sara A. Grimm, Humphrey Hung-Chang Yao Molecular Actions Underlying Wolffian Duct Regression in Sexual Differentiation of Murine Reproductive Tracts Sexual Development 2021-03-08 [PMID: 33684916]
Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2 Science Advances 2020-11-27 [PMID: 33239293]
Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2 Science Advances 2020-11-27 [PMID: 33239293]
L Wang, CM Cheng, J Qin, M Xu, CY Kao, J Shi, E You, W Gong, LP Rosa, P Chase, L Scampavia, F Madoux, T Spicer, P Hodder, HE Xu, SY Tsai, MJ Tsai Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment Sci Adv, 2020;6(18):eaaz8031. 2020 [PMID: 32494682] (ChIP, Human)	ChIP	Human
L Wang, CM Cheng, J Qin, M Xu, CY Kao, J Shi, E You, W Gong, LP Rosa, P Chase, L Scampavia, F Madoux, T Spicer, P Hodder, HE Xu, SY Tsai, MJ Tsai Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment Sci Adv, 2020-04-29;6(18):eaaz8031. 2020-04-29 [PMID: 32494682] (ChIP, Human)	ChIP	Human
Samir Mehanovic, Raifish E Mendoza-Villarroel, Robert S Viger, Jacques J Tremblay The Nuclear Receptor COUP-TFII Regulates Amhr2 Gene Transcription via a GC-Rich Promoter Element in Mouse Leydig Cells Journal of the Endocrine Society 2019-12-01 [PMID: 31723721]
SF Sorrells, MF Paredes, D Velmeshev, V Herranz-Pé, K Sandoval, S Mayer, EF Chang, R Insausti, AR Kriegstein, JL Rubenstein, J Manuel Gar, EJ Huang, A Alvarez-Bu Immature excitatory neurons develop during adolescence in the human amygdala Nat Commun, 2019-06-21;10(1):2748. 2019-06-21 [PMID: 31227709] (IHC, Human)	IHC	Human
S Bonney, BJC Dennison, M Wendlandt, JA Siegenthal Retinoic Acid Regulates Endothelial ?-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature Front Cell Neurosci, 2018-12-05;12(0):476. 2018-12-05 [PMID: 30568578] (IHC, Human)	IHC	Human
Seung-Kwon Ha, Govind Nair, Martina Absinta, Nicholas J. Luciano, Daniel S. Reich Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels BIO-PROTOCOL 2018-04-20 [PMID: 29780855]
Kim Brint Pedersen, Harshita Chodavarapu, Eric Lazartigues Forkhead Box Transcription Factors of the FOXA Class Are Required for Basal Transcription of Angiotensin-Converting Enzyme 2 Journal of the Endocrine Society 2017-04-01 [PMID: 29082356]
M Absinta, SK Ha, G Nair, P Sati, NJ Luciano, M Palisoc, A Louveau, KA Zaghloul, S Pittaluga, J Kipnis, DS Reich Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI Elife, 2017-10-03;6(0):. 2017-10-03 [PMID: 28971799] (IHC-P, Human, Primate)	IHC-P	Human, Primate
JJ Hwa, N Beckouche, L Huang, Y Kram, H Lindskog, RA Wang Abnormal arterial-venous fusions and fate specification in mouse embryos lacking blood flow Sci Rep, 2017-09-20;7(1):11965. 2017-09-20 [PMID: 28931948] (IHC, Mouse)	IHC	Mouse
JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations Cell Stem Cell, 2017-08-03;21(2):179-194.e4. 2017-08-03 [PMID: 28777944] (ICC, Human)	ICC	Human
R Smith, YT Huang, T Tian, D Vojtasova, O Mesalles-N, SM Pollard, T Pratt, DJ Price, V Fotaki The transcription factor Foxg1 promotes optic fissure closure in the mouse by suppressing Wnt8b in the nasal optic stalk J. Neurosci., 2017-07-20;0(0):. 2017-07-20 [PMID: 28729440] (IHC, Mouse)	IHC	Mouse
JL Close, Z Yao, BP Levi, JA Miller, TE Bakken, V Menon, JT Ting, A Wall, AR Krostag, ER Thomsen, AM Nelson, JK Mich, RD Hodge, SI Shehata, IA Glass, S Bort, NV Shapovalov, NK Ngo, JS Grimley, JW Phillips, CL Thompson, S Ramanathan, E Lein Single-Cell Profiling of an Inï¿½Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation Neuron, 2017-03-08;93(5):1035-1048.e5. 2017-03-08 [PMID: 28279351]
Man Chu, Taotao Li, Bin Shen, Xudong Cao, Haoyu Zhong, Luqing Zhang et al. Angiopoietin receptor Tie2 is required for vein specification and maintenance via regulating COUP-TFII eLife 2016-12-22 [PMID: 28005008]
Ayman Alzu'bi Distinct cortical and sub-cortical neurogenic domains for GABAergic interneuron precursor transcription factors NKX2.1, OLIG2 and COUP-TFII in early fetal human telencephalon Brain Struct Funct, 2016-11-30;0(0):. 2016-11-30 [PMID: 27905023] (Human)		Human
COUP-TFII regulates satellite cell function and muscular dystrophy J Clin Invest, 2016-09-12;0(0):. 2016-09-12 [PMID: 27617862] (ChIP, IHC, Mouse)	ChIP, IHC	Mouse
Diverse Functions of Retinoic Acid in Brain Vascular Development J Neurosci, 2016-07-20;36(29):7786-801. 2016-07-20 [PMID: 27445154] (IHC-Fr, Mouse)	IHC-Fr	Mouse
SC Lin, CY Kao, HJ Lee, CJ Creighton, MM Ittmann, SJ Tsai, SY Tsai, MJ Tsai Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer Nat Commun, 2016-04-25;7(0):11418. 2016-04-25 [PMID: 27108958] (Western Blot, Human)	Western Blot	Human
Tanwar V, Bylund J, Hu J, Yan J, Walthall J, Mukherjee A, Heaton W, Wang W, Potet F, Rai M, Kupershmidt S, Knapik E, Hatzopoulos A Gremlin 2 promotes differentiation of embryonic stem cells to atrial fate by activation of the JNK signaling pathway. Stem Cells, 2014-07-01;32(7):1774-88. 2014-07-01 [PMID: 24648383] (IHC, Mouse)	IHC	Mouse
Lindskog , Henrik, Kim , Yung Hae, Jelin , Eric B, Kong , Yupeng, Guevara-Gallardo , Salvador, Kim , Tyson N, Wang , Rong A Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals. Development, 2014-03-01;141(5):1120-8. 2014-03-01 [PMID: 24550118] (IHC, Mouse)	IHC	Mouse
Numan Al-Rayyan, Lacey M. Litchfield, Margarita M. Ivanova, Brandie N. Radde, Alan Cheng, Ahmed Elbedewy et al. 5-Aza-2-deoxycytidine and trichostatin A increase COUP-TFII expression in antiestrogen-resistant breast cancer cell lines Cancer Letters 2014-05-28 [PMID: 24513177]
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014-01-02;9(1):e84179. 2014-01-02 [PMID: 24392113] (ICC, Mouse)	ICC	Mouse
Litchfield L, Appana S, Datta S, Klinge C COUP-TFII inhibits NFkappaB activation in endocrine-resistant breast cancer cells. Mol Cell Endocrinol, 2013-10-17;382(1):358-67. 2013-10-17 [PMID: 24141032] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Jean-François Schmouth, Mauro Castellarin, Stéphanie Laprise, Kathleen G Banks, Russell J Bonaguro, Simone C McInerny et al. Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice BMC Biology 2013-12-01 [PMID: 24124870]
San-pin Wu, Chiang-Min Cheng, Rainer B. Lanz, Tiannan Wang, Jonathan L. Respress, Sameer Ather et al. Atrial Identity Is Determined by a COUP-TFII Regulatory Network Developmental Cell 2013-05-28 [PMID: 23725765]
Reema B. Davis, Carol D. Curtis, Courtney T. Griffin BRG1 promotes COUP-TFII expression and venous specification during embryonic vascular development Development 2013-03-15 [PMID: 23406903]
Mohammed H Endogenous Purification Reveals GREB1 as a Key Estrogen Receptor Regulatory Factor. Oncogene, 2013-02-09;3(2):342-9. 2013-02-09 [PMID: 23403292] (ChIP, Human)	ChIP	Human
Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG, Cai J, Pierce WM, Ivanova MM, Bates PJ, Appana SN, Datta S, Kulesza P, McBryan J, Young LS, Klinge CM Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor ÃŽÂ² transcription in breast cancer cells. PLoS ONE, 2012-05-31;7(5):e38278. 2012-05-31 [PMID: 22693611] (ICC, Human)	ICC	Human
Mayer S, Roeser M, Lachmann P Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression. J. Biol. Chem., 2012-05-29;287(29):24483-91. 2012-05-29 [PMID: 22645148] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
van den Driesche S, Walker M, McKinnell C, Scott HM, Eddie SL, Mitchell RT, Seckl JR, Drake AJ, Smith LB, Anderson RA, Sharpe RM Proposed role for COUP-TFII in regulating fetal Leydig cell steroidogenesis, perturbation of which leads to masculinization disorders in rodents. PLoS ONE, 2012-05-17;7(5):e37064. 2012-05-17 [PMID: 22615892] (IHC, Human, Rat)	IHC	Human, Rat
Rosa A, Brivanlou AH A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J., 2010-12-10;30(2):237-48. 2010-12-10 [PMID: 21151097] (Western Blot, Human)	Western Blot	Human
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 2010-04-01;120(5):1694-707. 2010-04-01 [PMID: 20364082] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
Aerbajinai W, Zhu J, Kumkhaek C, Chin K, Rodgers GP SCF induces gamma-globin gene expression by regulating downstream transcription factor COUP-TFII. Blood, 2009-04-28;114(1):187-94. 2009-04-28 [PMID: 19401563] (Western Blot, Human)	Western Blot	Human
Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M The transcription factor COUP-TFII is negatively regulated by insulin and glucose via Foxo1- and ChREBP-controlled pathways. Mol. Cell. Biol., 2008-09-02;28(21):6568-79. 2008-09-02 [PMID: 18765640] (Western Blot, Mouse)	Western Blot	Mouse
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COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]

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