LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

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Biological Strategies: Western Blot: LIM Kinase 1/2 [p Thr508] Antibody [NBP2-54645] - Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 ...read more
Immunohistochemistry-Paraffin: LIM Kinase 1/2 [p Thr508] Antibody [NBP2-54645] - Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).Bottom Image: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

LIM Kinase 1/2 [p Thr508] Antibody - BSA Free Summary

Immunogen
A peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1
Modification
p Thr508
Specificity
This antibody detects endogenous level of LIM Kinase 1/2 only when phosphorylated at threonine 508.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMK1
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Epitope affinity purified

Alternate Names for LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

  • EC 2.7.11.1
  • LIM domain kinase 1
  • LIM motif-containing protein kinase
  • LIMKLIMK-1

Background

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional LIM Kinase 1/2 Products

Array NBP2-54645

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Bioinformatics

Gene Symbol LIMK1