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Gelsolin/GSN Antibody (893205) [Unconjugated]

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Gelsolin/GSN Antibody was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
Gelsolin/GSN Antibody was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius ...read more
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse ...read more
Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (U‑87 MG) (NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A ...read more
Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Genetic Strategies: Western blot shows lysates of U2OS human osteosarcoma cell line and Gelsolin/GSN knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO, mIF
Clone
893205
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8170-SP
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Gelsolin/GSN Antibody (893205) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Gelsolin/GSN
Met1-Ala782
Accession # P06396
Specificity
Detects human Gelsolin/GSN in ELISAs. Detects human, mouse and rat Gelsolin/GSN in Western Blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Multiplex Immunofluorescence 20 ug/mL
  • Simple Western 5-20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
MAB8170 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gelsolin/GSN Antibody (893205) [Unconjugated]

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN

Background

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family.  Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm.  Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein.  Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis.  Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition.  Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gelsolin/GSN Antibody (MAB8170)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


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Bioinformatics

Gene Symbol GSN
Uniprot