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DNASE1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Analysis in human duodenum and liver tissues using Anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for ...read more
Western Blot: DNASE1 Antibody [NBP1-84999] - Analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] -Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] -Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] -Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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DNASE1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNASE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DNASE1 Protein (NBP1-84999PEP)
Publications
Read Publication using NBP1-84999.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNASE1 Antibody

  • deoxyribonuclease IFLJ38093
  • deoxyribonuclease-1
  • DKFZp686H0155
  • DNase I
  • DNase I, lysosomal
  • DNL1FLJ44902
  • Dornase alfa
  • DRNI
  • EC 3.1.21.1
  • human urine deoxyribonuclease I

Background

DNASE1, also known as Deoxyribonuclease 1, is an endonuclease found in the zymogen granules of the nuclear envelope. Responsible for DNA fragmentation and cell death during apoptosis, DNASE1 binds to G-actin and blocks actin polymerization. Mutations in DNASE1 cause systemic lupus erythematosus (SLE), an autoimmune disease causing inflammation and tissue damage. DNASE1 is also known to treat symptoms of cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNASE1 Antibody (NBP1-84999)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DNASE1 Antibody (NBP1-84999) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DNASE1