Home » GATA-1 » GATA-1 Antibodies » GATA-1 Antibody (4F5) - BSA Free
Novus Biologicals products are now on bio-techne.com

GATA-1 Antibody (4F5) - BSA Free

 (2 Publications)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, ...read more
Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ChIP
Clone
4F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-47492-0.025ml
100% Guarantee on every product

GATA-1 Antibody (4F5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human GATA-1 expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GATA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
Use in Chip reported in scientific literature (PMID:32763160)
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47492 in the following applications:
Publications
Read Publications using
NBP1-47492 in the following applications:

Reactivity Notes

Papio anubis reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for GATA-1 Antibody (4F5) - BSA Free

  • Eryf1
  • ERYF1GATA-binding protein 1 (globin transcription factor 1)
  • GATA binding protein 1 (globin transcription factor 1)
  • GATA1
  • GATA-1
  • GATA-1erythroid transcription factor
  • GATA-binding factor 1
  • GF-1
  • GF1globin transcription factor 1
  • NF-E1 DNA-binding protein
  • NFE1erythroid transcription factor 1
  • transcription factor GATA1
  • XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Nuclear Factor
Erythroid Derived 2 A ...
NBP1-82580
Immunohistochemistry-Paraffin Nuclear Factor Erythroid Derived 2 Antibody
Immunohistochemistry-Paraffin Nuclear Factor Erythroid Derived 2 Antibody
Immunohistochemistry-Paraffin Nuclear Factor Erythroid Derived 2 Antibody
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
QRSL Antibody
NBP1-87992
Western Blot QRSL Antibody
Western Blot QRSL Antibody
Immunohistochemistry-Paraffin QRSL Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SCL/Tal1 Antibody
(OTI5H1)
NBP2-01488
Western Blot SCL/Tal1 Antibody (OTI5H1)
Flow Cytometry SCL/Tal1 Antibody (OTI5H1)
Immunocytochemistry/ Immunofluorescence SCL/Tal1 Antibody (OTI5H1)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
GATA-2 Antibody
[Unconjugated]
AF2046
Western Blot GATA-2 Antibody [Unconjugated]
Simple Western GATA-2 Antibody [Unconjugated]
Western Blot GATA-2 Antibody [Unconjugated]
(11 Publications)
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
GATA-2 Antibody
[Unconjugated]
AF2046
Western Blot GATA-2 Antibody [Unconjugated]
Simple Western GATA-2 Antibody [Unconjugated]
Western Blot GATA-2 Antibody [Unconjugated]
(11 Publications)
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
Erythropoietin/EPO
[HRP]
MEP00B
N/A Erythropoietin/EPO [HRP]
N/A Erythropoietin/EPO [HRP]
(104 Publications)
Species: Mu
Applications: ELISA
ZFPM1 Antibody
NBP2-47572
Immunocytochemistry/ Immunofluorescence ZFPM1 Antibody
Immunohistochemistry-Paraffin ZFPM1 Antibody
Immunohistochemistry-Paraffin ZFPM1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Hemoglobin beta
Antibody
NBP2-14081
Immunocytochemistry/ Immunofluorescence Hemoglobin beta Antibody
Immunohistochemistry-Paraffin Hemoglobin beta Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
KLF1 Antibody
(1B6A3) - BSA Free
NBP2-37380
ELISA KLF1 Antibody (1B6A3) - BSA Free
Western Blot KLF1 Antibody (1B6A3) - BSA Free
Flow Cytometry KLF1 Antibody (1B6A3) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
SCLY Antibody
NBP2-48693
Immunohistochemistry-Paraffin SCLY Antibody
Immunocytochemistry/ Immunofluorescence SCLY Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Cytokeratin 7
Antibody (OV-TL12/30) ...
NBP2-47940
Simple Western Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free
Flow Cytometry Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free
Simple Western Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free
(1 Review)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
PU.1/Spi-1 Antibody
NBP2-27163
Western Blot PU.1/Spi-1 Antibody
Western Blot PU.1/Spi-1 Antibody
Flow Cytometry PU.1/Spi-1 Antibody
(1 Review)
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: Flow, WB
SP1 Antibody
NB600-232
Western Blot SP1 Antibody
Immunohistochemistry-Paraffin SP1 Antibody
Western Blot SP1 Antibody
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
PSG1 [HRP]
DPSG10
N/A PSG1 [HRP]
N/A PSG1 [HRP]
(2 Publications)
Species: Hu
Applications: ELISA
COCO/DAND5
[Unconjugated]
3047-CC
COCO/DAND5 [Unconjugated]
(4 Publications)
Species: Hu
Applications: BA
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
EPX Antibody
(rEPO104)
NBP3-07388
Immunohistochemistry-Paraffin EPX Antibody (rEPO104)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(204 Publications)
Species: Hu
Applications: BA
TIMP-1 [HRP]
DTM100
N/A TIMP-1 [HRP]
N/A TIMP-1 [HRP]
(96 Publications)
Species: Hu
Applications: ELISA
CD34 Antibody
[Unconjugated]
AF4117
Immunohistochemistry CD34 Antibody [Unconjugated]
(3 Reviews)
(37 Publications)
Species: Rt
Applications: IHC, WB

Publications for GATA-1 Antibody (NBP1-47492)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Chemotaxis, ICC/IF.


Filter By Application
Chemotaxis
(1)
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for GATA-1 Antibody (NBP1-47492) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Papio anubis.

Reviews using NBP1-47492:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Papio anubis
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GATA-1 NBP1-47492
Enlarge 		4
reviewed by:
Verified Customer
 IHC-P Papio anubis 02/26/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedfetal liver
SpeciesPapio anubis
Lot100706

Comments

CommentsIHC paraffin, species Papio anubis (Olive Baboon), fetal liver
Antigen retrieval 10mM sodium citrate, pH 6.0
Image-iT signal enhancer
Streptavidin/Biotin blocking kit
Primary Ab: 1:500 dilution
Second Ab (biotinylated): 1:200
Streptavidin-AlexaFluor488: 1:500 (green)
Quench autofluorescence with 1% Sudan Black in 70% methanol
Counterstain nuclei with propidium iodide (red)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GATA-1 Antibody (NBP1-47492) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GATA-1 Products

Research Areas for GATA-1 Antibody (NBP1-47492)

Find related products by research area.

Blogs on GATA-1

There are no specific blogs for GATA-1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
Immunohistochemistry-Paraffin GATA-1 NBP1-47492
Enlarge
 Verified Customer
02/26/2019
Application: IHC-P
Species: Papio anubis
read this review

Bioinformatics

Gene Symbol GATA1