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GATA-2 Antibody [Unconjugated]

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Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-2 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-2 Antigen Affinity-purified ...read more
Western blot shows lysates of LNCaP human prostate cancer cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse GATA-2 Antigen Affinity-purified ...read more
GATA‑2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 5 µg/mL ...read more
GATA‑2 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 5 µg/mL ...read more
GATA‑2 was detected in immersion fixed paraffin-embedded sections of human duodenum (blood vessel) using Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 1 ...read more
Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GATA‑2 at approximately 64 kDa (as indicated) using 5 µg/mL of Goat ...read more
Genetic Strategies: RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2046
Phycoerythrin
IC2046P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC2046P
Carrier Free
AF2046-SP
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GATA-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769
Specificity
Detects human and mouse GATA-2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF2046 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-2 Antibody [Unconjugated]

  • endothelial transcription factor GATA-2
  • FLJ45948
  • GATA binding protein 2
  • GATA2
  • GATA-2
  • GATA-binding protein 2NFE1B
  • MGC2306

Background

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).
  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-2 Antibody (AF2046)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, ICC, IHC, Western Blot.


Filter By Application
ChIP
(1)
ICC
(1)
IHC
(1)
Western Blot
(2)
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Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2046 Applications Species
Shao Xu, Sidong Wang, Timothy Theodore Ka Ki Tam, Pengtao Liu, Degong Ruan Derivation of trophoblast stem cells from human expanded potential stem cells STAR Protocols 2023-06-09 [PMID: 37300826]
Zemin Hou, Shengsong Huang, Zejie Mei, Longlong Chen, Jiacheng Guo, Yuanyuan Gao et al. Inhibiting 3 beta HSD1 to eliminate the oncogenic effects of progesterone in prostate cancer Cell Reports Medicine 2022-03-15 [PMID: 35492874]
Boksik Cha, Yen-Chun Ho, Xin Geng, Md. Riaj Mahamud, Lijuan Chen, Yeunhee Kim et al. YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling Development 2020-12-01 [PMID: 33060128]
Kiyohiko Sakata, Kana Fujimori, Satoru Komaki, Takuya Furuta, Yasuo Sugita, Kenji Ashida et al. Pituitary Gangliocytoma Producing TSH and TRH: A Review of “Gangliocytomas of the Sellar Region” The Journal of Clinical Endocrinology & Metabolism 2020-10-01 [PMID: 32706866]
V Muha, R Williamson, R Hills, AD McNeilly, TG McWilliams, J Alonso, M Schimpl, AC Leney, AJR Heck, C Sutherland, KD Read, RJ McCrimmon, SP Brooks, DMF van Aalten Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice Open Biol, 2019-11-27;9(11):190192. 11/27/2019 [PMID: 31771416]
Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126 Development 2019-11-01 [PMID: 31582413]
D Choi, E Park, E Jung, B Cha, S Lee, J Yu, PM Kim, S Lee, YJ Hong, CJ Koh, CW Cho, Y Wu, NL Jeon, AK Wong, L Shin, SR Kumar, I Bermejo-Mo, RS Srinivasan, IT Cho, YK Hong Piezo1 incorporates mechanical force signals to genetic program that governs lymphatic valve development and maintenance JCI Insight, 2019-03-07;0(0):. 2019-03-07 [PMID: 30676326] (ICC, Western Blot, Human) ICC, Western Blot Human
Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Dev Biol, 2015-11-02;409(1):218-33. 2015-11-02 [PMID: 26542011] (IHC, Mouse) IHC Mouse
Cortes-Lavaud X, Landecho M, Maicas M, Urquiza L, Merino J, Moreno-Miralles I, Odero M GATA2 germline mutations impair GATA2 transcription, causing haploinsufficiency: functional analysis of the p.Arg396Gln mutation. J Immunol, 2015-01-26;194(5):2190-8. 2015-01-26 [PMID: 25624456] (ChIP, Human) ChIP Human
Qiang Shi, Vida Hodara, Calvin R. Simerly, Gerald P. Schatten, John L. VandeBerg Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons Stem Cells and Development 2013-02-15 [PMID: 22931470]
Show All 11 Publications.

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Bioinformatics

Gene Symbol GATA2
Uniprot