![Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Western-Blot-NB100-2076-img0005.jpg "Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.")
| Reactivity | Hu, Bv, Fe, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | BA-4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Bovine alpha-Elastin. |
| Localization | Connective tissue. |
| Specificity | This recognizes insoluble elastin, alpha-elastin, soluble non-cross linked precursor of elastin (tropoelastin). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ELN |
| Purity | Ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30214669).. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:33091431). |
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| Storage | Store at 4C. |
| Buffer | Ascites |
| Preservative | 0.05% Sodium Azide |
| Purity | Ascites |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 11/04/2017 |
Summary
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_NB100-2076_8746.jpg)
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reviewed by:
Verified Customer |
IHC-P | Other | 07/07/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Elastin Antibody (NB100-2076)Find related products by research area.
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| Verified Customer 11/04/2017 |
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| Application: | IHC-Fr | |
| Species: | Mouse |
| Verified Customer 07/07/2014 |
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| Application: | IHC-P | |
| Species: | Other |
![Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human renal cell carcinoma stained with Elastin antibody (NB100-2076)](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0008.jpg "Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human renal cell carcinoma stained with Elastin antibody (NB100-2076)")
![Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0003.jpg "Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original magnification X100.](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0004.jpg "Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original magnification X100.")
![Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0006.jpg "Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.")
![Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin and GAGs following decellularization. Porcine vena cavas were decellularized and analyzed for ECM proteins. Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin and vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0220743), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0007.jpg "Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin and GAGs following decellularization. Porcine vena cavas were decellularized and analyzed for ECM proteins. Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin and vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0220743), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin & GAGs following decellurarization.Porcine vena cavas were decellularized & analyzed for ECM proteins. (A) Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin & vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 μm. (B-E) Quantification of ECM components with commercially available kits from 20–30 mg wet tissue (B) soluble collagen, (C) insoluble collagen, (D) elastin, & (E) GAGs. Fluorescence intensity of samples immunostained for (F) fibronectin & (G) vitronectin. The fluoresvence intensity was quantified with ImageJ, & the values were normalized to the untreated group. n = 5 for all groups. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31381614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2076_mouse-monoclonal-elastin-antibody-ba-4-31020241612629.jpg "Immunocytochemistry/ Immunofluorescence: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin & GAGs following decellurarization.Porcine vena cavas were decellularized & analyzed for ECM proteins. (A) Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin & vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 μm. (B-E) Quantification of ECM components with commercially available kits from 20–30 mg wet tissue (B) soluble collagen, (C) insoluble collagen, (D) elastin, & (E) GAGs. Fluorescence intensity of samples immunostained for (F) fibronectin & (G) vitronectin. The fluoresvence intensity was quantified with ImageJ, & the values were normalized to the untreated group. n = 5 for all groups. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31381614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
_NB100-2076_8746.jpg)