Laminin alpha 4 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha , beta , and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using AF3837	Applications	Species
Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C; Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis Cancers 2024-01-19 [PMID: 38275881] (IHC, Transgenic Mouse, Mouse)	IHC	Transgenic Mouse, Mouse
Arbaizar-Rovirosa, M;Gallizioli, M;Lozano, JJ;Sidorova, J;Pedragosa, J;Figuerola, S;Chaparro-Cabanillas, N;Boya, P;Graupera, M;Claret, M;Urra, X;Planas, AM; Transcriptomics and translatomics identify a robust inflammatory gene signature in brain endothelial cells after ischemic stroke Journal of neuroinflammation 2023-09-11 [PMID: 37691115] (IHC, Mouse, Transgenic Mouse)	IHC	Mouse, Transgenic Mouse
Jiekun Yang, Maria Vamvini, Pasquale Nigro, Li-Lun Ho, Kyriakitsa Galani, Marcus Alvarez et al. Single-cell dissection of the obesity-exercise axis in adipose-muscle tissues implies a critical role for mesenchymal stem cells Cell Metabolism 2022-10-04 [PMID: 36198295]
Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino et al. Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation Nature Immunology 2022-06-01 [PMID: 35618831]
Swathi Ayloo, Christopher Gallego Lazo, Shenghuan Sun, Wei Zhang, Bianxiao Cui, Chenghua Gu Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier Neuron 2022-05-01 [PMID: 35294899]
SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment Journal of Translational Medicine, 2022-02-02;20(1):62. 2022-02-02 [PMID: 35109875] (ICC/IF, Mouse)	ICC/IF	Mouse
M Nesbit, JC Mamo, M Majimbi, V Lam, R Takechi Automated Quantitative Analysis of ex vivo Blood-Brain Barrier Permeability Using Intellesis Machine-Learning Frontiers in Neuroscience, 2021-04-16;15(0):617221. 2021-04-16 [PMID: 33935625] (IHC, Mouse, Rat)	IHC	Mouse, Rat
Packer D, Martin PT. Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy(W) mouse model of MDC1A Mol Ther Methods Clin Dev 4/19/2021 [PMID: 33869655] Details: Citation using the FITC version of this antibody.
Emanuela Pasciuto, Oliver T. Burton, Carlos P. Roca, Vasiliki Lagou, Wenson D. Rajan, Tom Theys et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition Cell 2020-08-06 [PMID: 32702313]
B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis Sci Rep, 2019-12-17;9(1):19303. 2019-12-17 [PMID: 31848396] (IHC-P, Mouse)	IHC-P	Mouse
Kim J, Hopkinson M, Kavishwar M, Fernandez-Fuente M, Brown S Prenatal muscle development in a mouse model for the secondary dystroglycanopathies. Skelet Muscle, 2016-02-19;6(0):3. 2016-02-19 [PMID: 26900448] (IHC, Mouse)	IHC	Mouse
Francesc Miró-Mur, Isabel Pérez-de-Puig, Maura Ferrer-Ferrer, Xabier Urra, Carles Justicia, Angel Chamorro et al. Immature monocytes recruited to the ischemic mouse brain differentiate into macrophages with features of alternative activation Brain, Behavior, and Immunity 2016-03-01 [PMID: 26275369]
Yao Y, Chen Z, Norris E, Strickland S Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun, 2014-03-03;5(0):3413. 2014-03-03 [PMID: 24583950] (IHC, Mouse)	IHC	Mouse
Transforming growth factor β-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis. Am J Pathol, 2012-11-09;182(1):118-31. 2012-11-09 [PMID: 23142380] (IHC, Mouse)	IHC	Mouse
Flanagan K, Fitzgerald K, Baker J Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS. PLoS ONE, 2012-07-06;7(7):e40443. 2012-07-06 [PMID: 22792325] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ackroyd MR, Whitmore C, Prior S, Kaluarachchi M, Nikolic M, Mayer U, Muntoni F, Brown SC Fukutin-related protein alters the deposition of laminin in the eye and brain. J. Neurosci., 2011-09-07;31(36):12927-35. 2011-09-07 [PMID: 21900571] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 16 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	3 reviewed by: Bhavin Shah	Immunoflourescence in Frozen mouse brain sections	Mouse	11/25/2021	View Summary Application Other Sample Tested P12 20µm mouse brain frozen sections Species Mouse Lot YLCO120022 Comments Comments A lot of standardisation may be required for the antibody to work. good fixation of the tissue may help. for adult tissues, they need to be perfused well in order to achieve good results.

Array AF3837

• Laminin alpha 4 Antibodies
• Laminin alpha 4 ELISA Kit
• Laminin alpha 4 Proteins
• Laminin alpha 4 Proteins and Enzymes