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Laminin beta 3 Antibody (CL3353)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis in human skin and pancreas tissues using NBP2-46622 antibody. Corresponding LAMB3 RNA-seq data are ...read more
Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.
Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of A-431 cells using the Anti-LAMB3 monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. ...read more
Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis in human lung tissue
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of human prostate shows immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of human urinary bladder wall shows positivity in basement membrane of squamous epithelium.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of human fallopian tube shows immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of human tonsil shows absence of immunoreactivity in lymphoid tissue (negative control).
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining or rat rectum shows immunoreactivity in basement membrane of glandular epithelium and in blood vessels.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining or rat lung shows positivity in basement membrane of respiratory epithelium.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining or rat kidney shows immunoreactivity in renal glomerulus.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL3353
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Laminin beta 3 Antibody (CL3353) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence KLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSS mlGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAI mlRSADLTGLEKRVEQ
Epitope
ASEQALSAQEGFERI
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMB3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
Laminin beta 3 Recombinant Protein Antigen (NBP2-46622PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-46622 in the following applications:

Publications
Read Publications using
NBP2-46622 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Laminin beta 3 Antibody (CL3353)

  • BM600-125kDa
  • Epiligrin subunit bata
  • FLJ99565
  • Kalinin B1 chain
  • Kalinin subunit beta
  • kalinin-140kDa
  • LAM5
  • Laminin 5
  • Laminin B1k chain
  • laminin S B3 chain
  • laminin subunit beta-3
  • laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
  • laminin, beta 3
  • Laminin-5 subunit beta
  • LAMNB1
  • Nicein subunit beta
  • nicein-125kDa

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Laminin beta 3 Antibody (NBP2-46622)(2)

Review for Laminin beta 3 Antibody (NBP2-46622) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rabbit.

Reviews using NBP2-46622:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Apu Saikia
ICC Rabbit 05/04/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedRabbit tissue
SpeciesRabbit
Lot02999

Comments

Comments1 hour incubation at 1:500 dilution worked very well for immunohistochemistry

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin beta 3 Antibody (NBP2-46622) (0)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Apu Saikia
05/04/2018
Application: ICC
Species: Rabbit

Bioinformatics

Gene Symbol LAMB3
Entrez
Uniprot