![Immunocytochemistry/Immunofluorescence: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Staining Collagen XVII in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-SR46-05-Immunocytochemistry-Immunofluorescence-NBP2-67316-img0001.jpg "Immunocytochemistry/Immunofluorescence: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Staining Collagen XVII in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | SR46-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human Collagen XVII aa 1313-1420 / 1497. (SwissProt: Q9UMD9 Human; SwissProt: Q07563 Mouse; Unigene:40392 Rat) |
| Localization | Cell junction, Membrane, Secreted |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | COL17A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-67316 | Applications | Species |
|---|---|---|
| Fischer NG, Kobe AC, Dai J et al. Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices Acta biomaterialia 2021-12-29 [PMID: 34971784] (IF/IHC, Rat) | IF/IHC | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen XVII Antibody (NBP2-67316)Find related products by research area.
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| Gene Symbol | COL17A1 |
![Western Blot: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 ug/Lane)Lane 2: Mouse skin tissue lysate (20 ug/Lane)Lane 3: Rat skin tissue lysate (20 ug/Lane)Predicted band size: 148 kDaObserved band size: 130、180 kDaExposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-western-blot-26720249233..jpg "Western Blot: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 ug/Lane)Lane 2: Mouse skin tissue lysate (20 ug/Lane)Lane 3: Rat skin tissue lysate (20 ug/Lane)Predicted band size: 148 kDaObserved band size: 130、180 kDaExposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Human skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-2672024946..jpg "Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Human skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-26720249539..jpg "Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Rat skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-2672024970..jpg "Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Rat skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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