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![Immunocytochemistry/Immunofluorescence: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Staining Collagen XVII in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-SR46-05-Immunocytochemistry-Immunofluorescence-NBP2-67316-img0001.jpg "Immunocytochemistry/Immunofluorescence: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Staining Collagen XVII in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Western Blot: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 ug/Lane)Lane 2: Mouse skin tissue lysate (20 ug/Lane)Lane 3: Rat skin tissue lysate (20 ug/Lane)Predicted band size: 148 kDaObserved band size: 130、180 kDaExposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-western-blot-26720249233..jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Human skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-2672024946..jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] -Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-26720249539..jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Rat skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67316_rabbit-monoclonal-collagen-xvii-antibody-sr46-05-immunohistochemistry-paraffin-2672024970..jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Analysis in human skin and liver tissues. Corresponding COL17A1 RNA-seq data are represented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP1-91800-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Analysis in human skin and liver tissues. Corresponding COL17A1 RNA-seq data are represented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Collagen XVII Antibody [NBP1-91800] - Analysis in human cell lines A-431 and MCF-7 using anti-COL17A1 antibody. Corresponding COL17A1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Western-Blot-NBP1-91800-img0014.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP1-91800-img0019.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Staining of human skin shows moderate membranous positivity in basal layer of epidermal cells.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP1-91800-img0016.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP1-91800-img0017.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP1-91800] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP1-91800-img0018.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Analysis in human skin and liver tissues using NBP2-38686 antibody. Corresponding COL17A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP2-38686-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Analysis in human skin and liver tissues using NBP2-38686 antibody. Corresponding COL17A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP2-38686-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Staining of human Cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP2-38686-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP2-38686-img0007.jpg)
![Immunohistochemistry-Paraffin: Collagen XVII Antibody [NBP2-38686] - Staining of human Skin shows weak granular cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-Immunohistochemistry-Paraffin-NBP2-38686-img0009.jpg)
![Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485] - Western blot analysis of extracts of A-431 cells, using Collagen XVII Rabbit mAb (NBP3-16485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-8W2C8-Western-Blot-NBP3-16485-img0002.jpg "Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485] - Western blot analysis of extracts of A-431 cells, using Collagen XVII Rabbit mAb (NBP3-16485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Collagen XVII Antibody (8W2C8) [NBP3-16485] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Collagen XVII Rabbit mAb (NBP3-16485) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-XVII-Antibody-8W2C8-Immunohistochemistry-Paraffin-NBP3-16485-img0001.jpg)
![ELISA: Human Collagen XVII ELISA Kit (Chemiluminescence) [NBP2-75836] - Samples were spiked with high concentrations of Human Collagen XVII and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75836_human-collagen-xvii-elisa-kit-chemiluminescence-13220241685342.jpg "ELISA: Human Collagen XVII ELISA Kit (Chemiluminescence) [NBP2-75836] - Samples were spiked with high concentrations of Human Collagen XVII and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Collagen XVII ELISA Kit (Chemiluminescence) [NBP2-75836] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75836_human-collagen-xvii-elisa-kit-chemiluminescence-432024857456.jpg)
![ELISA: Human Collagen XVII ELISA Kit (Colorimetric) [NBP2-75837] - Samples were spiked with high concentrations of Human Collagen XVII and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75837_human-collagen-xvii-elisa-kit-colorimetric-132202416215331.jpg "ELISA: Human Collagen XVII ELISA Kit (Colorimetric) [NBP2-75837] - Samples were spiked with high concentrations of Human Collagen XVII and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Collagen XVII ELISA Kit (Colorimetric) [NBP2-75837] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75837_human-collagen-xvii-elisa-kit-colorimetric-432024859721.jpg)
![Western Blot: Collagen XVII Overexpression Lysate (Adult Normal) [NBL1-09350] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen XVII.](http://images.novusbio.com/fullsize/Collagen-XVII-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09350-img0002.jpg "Western Blot: Collagen XVII Overexpression Lysate (Adult Normal) [NBL1-09350] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen XVII.")
![Western Blot: Collagen XVII Antibody (15G9) [NBP3-26647] - Positive Western Blot detected in: HEPG2 whole cell lysate. All lanes: Collagen XVII Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 150 kDa Observed band size: 150 kDa](http://images.novusbio.com/fullsize/nbp3-26647_rabbit-collagen-xvii-mab-15g9-28220241148587.jpg "Western Blot: Collagen XVII Antibody (15G9) [NBP3-26647] - Positive Western Blot detected in: HEPG2 whole cell lysate. All lanes: Collagen XVII Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 150 kDa Observed band size: 150 kDa")