HDAC1 Antibody

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, KD, Simple Western, WB.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using NB100-56340	Applications	Species
Kappert L, Ruzicka P, Kutikhin A Et al. Loss of Nfat5 promotes lipid accumulation in vascular smooth muscle cells FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-09-01 [PMID: 34383982] (WB, Mouse)	WB	Mouse
Lazarchuk P, Hernandez-Villanueva J, Pavlova Mn Et Al. Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells Mol. Cell. Biol. 2020-02-03 [PMID: 32015101] (WB, KD, ICC/IF)	WB, KD, ICC/IF
Wijdeven RH, van Luijn MM, Wierenga-Wolf AF et al. Chemical and genetic control of IFNg-induced MHCII expression. EMBO Rep. 2018-07-18 [PMID: 30021835] (WB, Human)	WB	Human
Laumet G, Garriga J, Chen SR et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat. Neurosci. 2015-12-01 [PMID: 26551542] (WB, IHC-Fr, Rat)	WB, IHC-Fr	Rat
Matthias Patrick. Too much or too little, how much HDAC activity is good for you? Blood. 2013-03-14 [PMID: 23493769] (WB)	WB
Johnson AM The Role of the Tumor Microenvironment on the Progression of Lung Cancer Thesis 2020-01-01 (WB, Mouse, Human)	WB	Mouse, Human
Laban H, Siegmund S, Zappe M et al. NFAT5/TonEBP Limits Pulmonary Vascular Resistance in the Hypoxic Lung by Controlling Mitochondrial Reactive Oxygen Species Generation in Arterial Smooth Muscle Cells Cells 2021-11-24 [PMID: 34943801]
Alvarez MEG, McGuire BC, Keating AF Obesity alters the ovarian proteomic response to zearalenone exposure Biology of reproduction 2021-04-14 [PMID: 33855340]
Zhang, Q;Chao, TC;Patil, VS;Qin, Y;Tiwari, SK;Chiou, J;Dobin, A;Tsai, CM;Li, Z;Dang, J;Gupta, S;Urdahl, K;Nizet, V;Gingeras, TR;Gaulton, KJ;Rana, TM; The long noncoding RNA ROCKI regulates inflammatory gene expression EMBO J. 2019-04-15 [PMID: 30918008] (WB, Human)	WB	Human
Heideman Marinus R, Lancini Cesare, Proost Natalie et al. Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis. Haematologica. 2014-08-01 [PMID: 24763403] (WB, Mouse)	WB	Mouse
Vlaming H, McLean C, Korthout T, Alemdehy MF. Evolutionarily-conserved chromatin crosstalk generates a DOT1L-dose dependency in thymic lymphoma caused by loss of HDAC1 bioRxiv 2019-01-10 (WB, Mouse)	WB	Mouse
Zimberlin CD, Lancini C, Sno R et al. HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis FASEB J. 2015-02-03 [PMID: 25648995] (IHC-P, Mouse) Details: HDAC1 antibody used for IHC-P application on intestine of WT, HOM;HET, and DKO mice, 4 /8 days after stimulation with beta naphthoflavone/b-NF (Figure 1B and 1F). The IHC-P involved - 24 hours tissue fixation in ethanol-acetic acid-formol saline fixative, blocking with goat serum for 30 minutes, overnight incubation with primary antibody, detection with HRP conjugated primary and DAB. The antibody also used for immuno-cytochemistry staining (DAB detection) of DKO mouse organoids subjected to control or beta-NF treatment in vitro for 72 hours (Figure 3B).	IHC-P	Mouse
Heideman MR, Wilting RH, Yanover E et al. Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function. Blood. 2013-03-14 [PMID: 23327920]
Wilson AJ, Byun DS, Nasser S et al. HDAC4 promotes growth of colon cancer cells via repression of p21. Mol Biol Cell. 2008-10-01 [PMID: 18632985] (WB) Details: Human HTC116 cells: 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10A,C,D,H.	WB
Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006-05-12 [PMID: 16533812]
Laban H, Sigmund S, Schlereth K et al. NFAT5-dependent transcriptional stress control of endothelial cells prevents maladaptive remodeling of pulmonary arterioles in the hypoxic lung bioRxiv 2023-10-22 (Simple Western, Mouse) Details: Dilution 1:10	Simple Western	Mouse
Opichka M Placental and Syncytiotrophoblast-Specific GAQ Signaling in the Pathogenesis of Preeclampsia Thesis 2023-01-01 (Simple Western)	Simple Western
Huang Hui, Dawicki Wojciech, Zhang Xiaobei et al. Tolerogenic dendritic cells induce CD4+CD25hiFoxp3+ regulatory T cell differentiation from CD4+CD25-/loFoxp3- effector T cells. J Immunol. 2010-11-01 [PMID: 20870943] (IF/IHC, Mouse)	IF/IHC	Mouse
Wilting Roel H, Yanover Eva, Heideman Marinus R et al. Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis. EMBO J. 2010-08-04 [PMID: 20571512] (IF/IHC)	IF/IHC
Show All 19 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Aileen Keating	WB	Pig	11/10/2016	View Summary Application Western Blot Sample Tested Ovary tissue Species Pig Comments Comments -Blocked in 5% BSA .2% PBST for 4 hours -Primary was 1:250 in 5% BSA .2% PBST overnight at 4C -Secondary was goat x rabbit 1:1000 in 5% BSA .2% PBST 1 hour
	5 reviewed by: Verified Customer	WB	Mouse	12/12/2014	View Summary Application Western Blot Sample Tested See PMID 23327920 Species Mouse

Array NB100-56340

• HDAC1 Antibodies
• HDAC1 ELISA Kits
• HDAC1 Kit
• HDAC1 Lysate
• HDAC1 Proteins