HDAC3 Antibody

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Chemotaxis, ICC/IF, IHC-P, IP, WB.

Showing Publications 1 - 9 of 9.

Publications using NB100-1669	Applications	Species
Wang J, Liu R, Wang Y Et Al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression J Cancer 2021-08-02 [PMID: 34335948] (Chemotaxis)	Chemotaxis
Baek, M H, Park, J Y Et al. The combination of histone deacetylase and p53 expressions and histological subtype has prognostic implication in uterine leiomyosarcoma. Jpn J Clin Oncol 2019-08-01 [PMID: 31329907] (IP, Mouse)	IP	Mouse
Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. 2016-05-01 [PMID: 27127168] (IHC-P, Human)	IHC-P	Human
Mano T, Suzuki T, Tsuji S, Iwata A. Differential Effect of HDAC3 on Cytoplasmic and nuclear Huntingtin Aggregates PLoS OnE et al. 2014-11-08 [PMID: 25380050] (ICC/IF, WB, Human) Details: HDAC3 antibody used for WB on HeLa derived E3 or n3 cells transfected or not with shRnA HDAC3 (Figure 2). Antibody also used for ICC-IF on 4% paraformaldehyd fixed E3 or n3 cells - HDAC3 associates exclusively with nuclear inclusion bodies (Figure 4C)	ICC/IF, WB	Human
Wilson AJ, Byun DS, Nasser S et al. HDAC4 promotes growth of colon cancer cells via repression of p21. Mol Biol Cell. 2008-10-01 [PMID: 18632985] Details: Human HTC116 cells: 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10A,C,D,H.
Jiang Chunling, Zhou Bingsen, Fan Kenneth et al. A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells. Anticancer Res. 2007-11-01 [PMID: 18225533] (WB, Human)	WB	Human
Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006-05-12 [PMID: 16533812]
Imbriano C, Gurtner A, Cocchiarella F et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol. 2005-05-01 [PMID: 15831478] (Chemotaxis)	Chemotaxis
Caretti G, Salsi V, Vecchi C et al. Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters. J Biol Chem. 2003-08-15 [PMID: 12771133] (Chemotaxis) Details: ChIP, Fig. 3	Chemotaxis

Array NB100-1669

• HDAC3 Antibodies
• HDAC3 ELISA Kits
• HDAC3 Lysate
• HDAC3 Proteins