Jak2 Antibody

Images

 
Western Blot: Jak2 Antibody [NBP1-22975] - Whole cell lysate from Jurkat (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAK2 antibody used for ...read more
Immunohistochemistry-Paraffin: Jak2 Antibody [NBP1-22975] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAK2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Jak2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1082 and 1132 of human Janus kinase 2 using the numbering given in entry NP_004963.1 (GeneID 3717).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JAK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Jak2 Antibody

  • EC 2.7.10
  • EC 2.7.10.2
  • Jak2
  • JAK-2
  • Janus kinase 2Janus kinase 2 (a protein tyrosine kinase)
  • JTK10
  • tyrosine-protein kinase JAK2

Background

JAK2 (Janus kinase 2) was originally cloned via screening of mouse cDNA libraries with PCR generated probes representing highly conserved elements in the protein tyrosine kinase (PTK) family. JAK2, as well as JAK, was named after the Roman god, Janus, the god of gates or doors, and who is depicted as having two faces which face opposite directions. JAK kinases bear two nearly identical phosphate transferring domains and function to transduce signals from type I and type II cytokine receptors for the activation of STAT (signal transducers and activators of transcription) transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1799
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
MAB4260
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
AF2168
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
AF1584
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
MEP00B
Species: Mu
Applications: ELISA
NB400-141
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
AF2894
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
M6000B
Species: Mu
Applications: ELISA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
AF1360
Species: Mu
Applications: IHC, WB
NBP2-37737
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, WB
203-IL
Species: Hu
Applications: BA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
AF1016
Species: Hu
Applications: Neut, Simple Western, WB
MAB3427
Species: Hu
Applications: WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
DTM100
Species: Hu
Applications: ELISA
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB

Publications for Jak2 Antibody (NBP1-22975) (0)

There are no publications for Jak2 Antibody (NBP1-22975).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Jak2 Antibody (NBP1-22975) (0)

There are no reviews for Jak2 Antibody (NBP1-22975). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Jak2 Antibody (NBP1-22975) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Jak2 Products

Array NBP1-22975

Research Areas for Jak2 Antibody (NBP1-22975)

Find related products by research area.

Blogs on Jak2.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Jak2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol JAK2
Entrez
Uniprot