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Jak3 Antibody (5H2) - BSA Free

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Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Immunocytochemistry/ Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more
Flow Cytometry: Jak3 Antibody (5H2) [NBP2-37737] - Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
Immunocytochemistry/ Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37737-0.025ml
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Jak3 Antibody (5H2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Jak3 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
JAK3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-37737 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Jak3 Antibody (5H2) - BSA Free

  • EC 2.7.10
  • Jak3
  • JAK3_HUMAN
  • JAK-3EC 2.7.10.2
  • JAKL
  • JAKLtyrosine-protein kinase JAK3
  • Janus kinase 3Leukocyte janus kinase
  • LJAK
  • L-JAKJanus kinase 3 (a protein tyrosine kinase, leukocyte)

Background

Jak3 (Janus Kinase 3) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK3. JAK3 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system, stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Jak3 Antibody
(5H2) - BSA Free
NBP2-37737
Jak3 Antibody (5H2) - BSA Free
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF

Publications for Jak3 Antibody (NBP2-37737)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Jak3 Antibody (NBP2-37737) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Jak3.

Check out the latest blog posts on Jak3.
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
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Bioinformatics

Gene Symbol JAK3