IKK gamma Antibody (72C627)

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg. This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 7 of 7.

Publications using NB100-56542	Applications	Species
Konrath F, Witt J, Sauter TK, Kulms D. Identification of new IkBa complexes by an iterative experimental and mathematical modeling approach. PLoS Comput. Biol. 2014-03-01 [PMID: 24675998] (WB, Human) Details: kB epithelial carcinoma cell line, Fig 5E-G. The specificity of the Ikkgamma mAb was validated with an IP/WB assay by showing in WB that it recognized Ikkgamma IP'd from kB cells with a different Ikkgamma antibody (Fig 5G).	WB	Human
Ren T, Takahashi Y, Liu X et al. HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains. Oncogene 2013-12-23 [PMID: 24362528] (WB, Human)	WB	Human
Siggs OM, Berger M, Krebs P et al. A mutation of Ikbkg causes immune deficiency without impairing degradation of IkappaB alpha. Proc Natl Acad Sci U S A. 2010-02-16 [PMID: 20133626] Details: NEMO (IMG-324A): WB (mouse macrophage lysates), Fig 2C.
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol 2010-05-01 [PMID: 20103608] Details: This citation used the Alexa Fluor 488 version of this antibody.
Lamothe B, Campos AD, Webster WK et al. The RING domain and first zinc finger of TRAF6 coordinate signaling by interleukin-1, lipopolysaccharide, and RANKL. J Biol Chem. 2008-09-05 [PMID: 18617513] (WB, Mouse)	WB	Mouse
Wunderlich FT, Luedde T, Singer S et al. Hepatic NF-kappa B essential modulator deficiency prevents obesity-induced insulin resistance but synergizes with high-fat feeding in tumorigenesis. Proc Natl Acad Sci U S A. 2008-01-29 [PMID: 18216263] (WB)	WB
Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003-05-01 [PMID: 12771574] Details: Products cited for western blot in male Sprague-Dawley rats ischemic cortex, ischemic subcortex, contralateral cortex, and contralateral subcortex tissue. The rats were subject to mild and delayed hypothermia. 1. IKK-gamma (IMG-324A): Fig 4A & 4B. 2. IkB-

Array NB100-56542

• IKK gamma Antibodies
• IKK gamma Inhibitor
• IKK gamma Lysates
• IKK gamma Proteins