Histone H4 [ac Lys16] Antibody - BSA Free

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 3 of 3.

Publications using NB21-2077	Applications	Species
Rishi JK, Timme K, White HE et al. Obesity partially potentiates dimethylbenz[a]anthracene-exposed ovotoxicity by altering the DNA damage repair response in mice� Biology of reproduction 2023-01-25 [PMID: 36702632] (WB, Mouse) Details: Dilution used in WB 1:500	WB	Mouse
Alvarez MEG, McGuire BC, Keating AF Obesity alters the ovarian proteomic response to zearalenone exposure Biology of reproduction 2021-04-14 [PMID: 33855340]
Gonzalez-Alvarez ME Investigating the Ovarian Impacts of Zearalenone Exposure and Influence of Altered Body Composition on Ovotoxicity Thesis 2021-01-01

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Pig	11/17/2016	View Summary Application Western Blot Sample Tested Ovary tissue Species Pig Comments Comments Sample Information:      Cell Line or Tissue: Ovary      Species: Pig      Treatment: None. Run on control tissue Controls:      Positive Control: None      Negative Control: Primary only, secondary only, and secondary with IgG all negative      Loading Control (please attach additional images if applicable): Ponceau S stain Total Protein Loaded: 40 ug/well Electrophoresis: Gel Percentage: Gradient, 4-20%      Voltage: 50V then 90V      Time: 5 min then 1 hr Membrane Transfer:      Method (Submersion/Semi-dry): Semi-dry using iBlot2 system      Membrane Type (PVDF/Nitrocellulose): Nitrocellulose      Time: 7 min (iBlot2 Protocol 0)      Voltage: Click here to enter text. Blocking:      Blocking Solution: 5% BSA in .2% PBST      Time: 1.5 hours Primary Antibody:      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST           Incubation Time: overnight           Incubation Temperature: 4°C Washing Conditions:      Wash Solution: .2% PBST      Time and Repetitions: 3X, 10 minutes each Secondary Antibody Manufacturer and Catalog #: Cell Signaling, #7074 Secondary description: goat anti-rabbit      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST      Incubation Time: 1 hour      Incubation Temperature: room temperature Detection Method: Detection: SignalFire ECL reagent (Cell Signaling) Procedure: ECL on blot 3 min Development Time: 5 min Molecular weight of band(s): 13 kDa

Array NB21-2077

• Histone H4 Antibodies
• Histone H4 Lysate
• Histone H4 Proteins