![Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-44234-img0002.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.")
| Reactivity | YeSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Hexokinase 2 Antibody was produced by repeated immunizations with yeast hexokinase protein. (Uniprot: P04806) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HK2 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by western blotting and for ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below. |
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| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
Research Areas for Hexokinase 2 Antibody (NBP2-44234)Find related products by research area.
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![ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-ELISA-NBP2-44234-img0001.jpg "ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.")
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