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Hexokinase 1 Antibody (ST47-05)

Images

 
Western Blot: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of Hexokinase 1 on different lysates using anti-Hexokinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7 ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded mouse skin tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
Flow Cytometry: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Flow cytometric analysis of K562 cells with Hexokinase 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded human kidney tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded human lung tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded mouse brain tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded mouse colon tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
ST47-05
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Hexokinase 1 Antibody (ST47-05) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Hexokinase 1 aa 100-140. (SwissProt: P19367 Human; SwissProt: P17710 Mouse; SwissProt: P05708 Rat)
Localization
Mitochondrion outer membrane, Cytoplasm, Membrane, Mitochondrion.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-67503 in the following applications:

Publications
Read Publication using NBP2-67503.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Hexokinase 1 Antibody (ST47-05)

  • Brain form hexokinase
  • EC 2.7.1
  • EC 2.7.1.1
  • glycolytic enzyme
  • Hexokinase 1
  • Hexokinase type I
  • hexokinase-1
  • HK I
  • HK1
  • HK1-ta
  • HK1-tb
  • HK1-tc
  • HKI
  • HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hexokinase 1 Antibody (NBP2-67503)(1)

Review for Hexokinase 1 Antibody (NBP2-67503) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NBP2-67503:
Filter by Applications
WB
(1)
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Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Hexokinase 1 NBP2-67503
Enlarge
5
reviewed by:
Verified Customer
WB Human and Mouse 01/13/2020
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate,Liver cancer tissue
SpeciesHuman and Mouse
LotHL0222

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hexokinase 1 Antibody (NBP2-67503) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
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1
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Verified Customer
01/13/2020
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol HK1