![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0021.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | OTI4C5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell. |
| Specificity | Human Hexokinase-2 protein is 74% identical with Hexokinase-1 and our Hexokinase 2 antibody (clone 4C5) is not expected to crossreact with Hexokinase-1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HK2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 102.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Hexokinase 2 Antibody (NBP2-02272)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0012.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.")
![Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Flow-Cytometry-NBP2-02272-img0019.jpg "Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.")
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0017.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.")
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0018.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0020.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")