Home » Protein S/PROS1 » Protein S Antibodies » Protein S/PROS1 Antibody [Unconjugated]

Protein S/PROS1 Antibody [Unconjugated]

 (7 Publications)
 
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Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG ...read more
Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4036-SP
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Protein S/PROS1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protein S
Ala42-Trp670
Accession # P07225
Specificity
Detects human PROS1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PROS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF4036 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein S/PROS1 Antibody [Unconjugated]

  • EC 3.4.21
  • PROS
  • PROS1
  • protein S (alpha)
  • Protein S
  • protein Sa
  • PS21
  • PS22
  • PS23
  • PS24
  • PS25
  • PSA
  • vitamin K-dependent plasma protein S
  • vitamin K-dependent protein S

Background

Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42‑87), four EGF-like repeats (aa 117-283) and two laminin G-like domains (aa 299‑666). Over aa 42-670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protein S/PROS1
Antibody [Unconjugate
AF4036
Protein S/PROS1 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western

Publications for Protein S/PROS1 Antibody (AF4036)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(1)
Western Blot
(3)
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Human
(1)
Mouse
(3)
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Showing Publications 1 - 7 of 7.
Publications using AF4036 Applications Species
ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435] (Western Blot, Mouse) Western Blot Mouse
BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 2018-01-02;515(0):176-190. 2018-01-02 [PMID: 29304470] (Western Blot, Human) Western Blot Human
Christine Baumann, Axel Ullrich, Robert Torka GAS6-expressing and self-sustaining cancer cells in 3D spheroids activate the PDK-RSK-mTOR pathway for survival and drug resistance Molecular Oncology 2017-10-01 [PMID: 28675785]
Wang ZH, Zhao ZJ, Xu K et al. Hereditary protein S deficiency leads to ischemic stroke. Mol Med Rep 9/1/2015 [PMID: 25997409]
Zagorska A, Traves P, Lew E, Dransfield I, Lemke G Diversification of TAM receptor tyrosine kinase function. Nat Immunol, 2014-09-07;15(10):920-8. 2014-09-07 [PMID: 25194421] (Western Blot, Mouse) Western Blot Mouse
Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis. Neuron, 2012-12-20;76(6):1123-32. 12/20/2012 [PMID: 23259948]
Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010-03-26;115(23):4963-72. 2010-03-26 [PMID: 20348395] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol PROS1
Uniprot