![Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Western-Blot-NBP2-20881-img0001.jpg "Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary. |
| Localization | Prevacuolar compartment membrane; Peripheral membrane protein; Late endosome membrane; Peripheral membrane protein (Probable) |
| Predicted Species | Mouse (96%), Rat (96%), Rhesus Macaque (99%), Bovine (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VPS4B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Intracellular Staining by Flow Cytometry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Flow_Cytometry_11328.jpg)
![Simple Western Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin1_AF1152_Simple_Western_16673.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for VPS4B Antibody (NBP2-20881)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20881-img0004.jpg "Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min .](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-20881-img0005.jpg "Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min .")
![Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20881-img0003.jpg "Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")